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master's thesis
Probiotička svojstva soja Lactobacillus brevis SF15b koji eksprimira S-proteine
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:094596
Šantak, Manuela
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Šantak, M. (2016). Probiotička svojstva soja Lactobacillus brevis SF15b koji eksprimira S-proteine (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:094596

Šantak, Manuela. "Probiotička svojstva soja Lactobacillus brevis SF15b koji eksprimira S-proteine." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:094596

Šantak, Manuela. "Probiotička svojstva soja Lactobacillus brevis SF15b koji eksprimira S-proteine." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:094596

Šantak, M. (2016). 'Probiotička svojstva soja Lactobacillus brevis SF15b koji eksprimira S-proteine', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:094596

Šantak M. Probiotička svojstva soja Lactobacillus brevis SF15b koji eksprimira S-proteine [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:094596

M. Šantak, "Probiotička svojstva soja Lactobacillus brevis SF15b koji eksprimira S-proteine", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:094596

Metadata
TitleProbiotička svojstva soja Lactobacillus brevis SF15b koji eksprimira S-proteine
Title (english)Probiotic characteristics of strain Lactobacillus brevis SF15b expressing S-proteins
AuthorManuela Šantak
MentorJagoda Šušković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2016-09-23, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Probiotici su živi mikroorganizmi koji, kada su prisutni u odgovarajućem broju, poboljšavaju zdravlje domaćina. Bakterije mliječne kiseline, među kojima se posebno ističu bakterije iz roda Lactobacillus, su najbolji modeli za probiotičku upotrebu te karakterizaciju pozitivnih učinaka. Cilj ovog rada bio je ispitati probiotička svojstva soja Lactobacillus brevis SF15B koji eksprimira S-proteine. Pritom se kao pozitivna kontrola koristio soj L. helveticus M92 koji eksprimira S-proteine, dok je negativna kontrola bio soj L. plantarum D13 koji navedene proteine ne eksprimira. Osim S-proteina, na površini stanica ispitivanog soja nalaze se i SLAP (Eng. Surface-Layer Associated Proteins). Uloga S-proteina u probiotičkom djelovanju soja SF15B ispitana je iz aspekta adhezije na mucin, prilikom čega je dokazano da prisutnost sloja S-proteina pridonosi boljim adhezijskim svojstvima. Osim toga, soj L. brevis SF15B ima sposobnost preživljavanja u pojedinim nepovoljnim uvjetima koji mogu vladati u ljudskom gastrointestinalnom traktu te pokazuje značajnu proteolitičku aktivnost.
Abstract (english)
Probiotics are live microorganisms which, when administered in adequate amount, confer a health benefit on the host. Lactic acid bacteria, especially bacteria of the genus Lactobacillus, are of first choice microorganisms for probiotic application and characterization of their effect on human health. The aim of this study was to examine probiotic properties of the strain Lactobacillus brevis SF15B which expresses S-layer proteins. Strain L. helveticus M92, which also contains layer of S-proteins on the surface of the bacterial cells, was used as positive control, while strain L. plantarum D13, which doesn`t express mentioned proteins, was used as negative control. Besides S-layer proteins, on the cell surface of strain SF15B there are also SLAPs (Eng. Surface-Layer Associated Proteins). The role of S-layer proteins in probiotic activity of strain SF15B was examined from the aspect of adhesion to mucin, during which it was proven that the presence of S-layer proteins contributes to better adhesion properties. In addition, strain L. brevis SF15B has the ability to survive in the various rigorous conditions which are present in the human gastrointestinal tract and exhibits significant proteolytic activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:094596
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-24 11:39:56