prikaz prve stranice dokumenta Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka
  Download
PDF 707.41 KB
undergraduate thesis
Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:544788
Lindić, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Lindić, P. (2017). Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:544788

Lindić, Petra. "Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:544788

Lindić, Petra. "Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:544788

Lindić, P. (2017). 'Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 09 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:544788

Lindić P. Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 December 09] Available at: https://urn.nsk.hr/urn:nbn:hr:159:544788

P. Lindić, "Izolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:544788

Metadata
TitleIzolacija DNA iz različitih uzoraka tkiva divljih (Felis silvestris Schreber, 1777) i domaćih (Felis catus Linnaeus, 1758) mačaka
Title (english)DNA extraction from different tissue samples of wild (Felis silvestris Schreber, 1777) and domestic (Felis catus Linnaeus, 1758) cat
AuthorPetra Lindić
MentorReno Hrašćan (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2017-07-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Izolacija DNA je uklanjanje deoksiribonukleinske kiseline iz svih stanica koje se nalaze u određenom uzorku. Za izolaciju DNA korišten je komplet QIAamp® DNA Mini (Hilden, Njemačka), a sama izolacija izvršena je prema protokolu proizvođača, koji se razlikuje za različite vrste uzoraka. Pomoću tog kompleta izolirana je genomska DNA, tj. DNA koja se ne nalazi samo u jezgrama stanica već i u mitohondrijima. Istraživanje je provedeno na 21 uzorku divljih i 47 uzoraka domaćih mačaka. Uzorci iz kojih se izolirala DNA su: mišićna tkiva divljih mačaka, krv, slina i dlaka domaćih mačaka. Na kraju uspješne izolacije dobiveno je 300 ili 400 μl (ovisno o uzorku iz kojeg se izolira) otopine genomske DNA. Najuspješnije izolacije dobivene su iz krvi i sline, zatim iz mišićnog tkiva, a najneuspješnija izolacija je iz dlake. Od svih vrsta tkiva jedino iz dlake nije niti jednom uspješno izolirana DNA.
Abstract (english)
DNA extraction is the removal of deoxyribonucleic acid from all cells in a particular sample. For DNA extraction, QIAamp® DNA Mini kit (Hilden, Deutschland) is used and extraction was performed according to the manufacturer's protocol, which differs for different types of samples. With this kit genomic DNA is extracted, i.e. DNA that is not only found in the cell nuclei but also in the mitochondria. The study was conducted on 21 wild and 47 domestic cat samples. Samples from which DNA was extracting are: muscle tissue of wild cats, blood, slime and hair of domestic cats. At the end of successful extraction, 300 or 400 μl (depending on the sample from which it is extracted) solution of genomic DNA is obtained. The most successful extractions are obtained from blood and saliva, then from muscle tissue and the most unsuccessful extraction is from the hair. Of all tissue types, only from the hair DNA wasn’t extracted.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:544788
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-07-19 08:11:32