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undergraduate thesis
Površinski proteini Lactobacillus vrsta
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:941999
Puljić, Dominika
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Puljić, D. (2017). Površinski proteini Lactobacillus vrsta (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:941999

Puljić, Dominika. "Površinski proteini Lactobacillus vrsta." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:941999

Puljić, Dominika. "Površinski proteini Lactobacillus vrsta." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:941999

Puljić, D. (2017). 'Površinski proteini Lactobacillus vrsta', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 22 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:941999

Puljić D. Površinski proteini Lactobacillus vrsta [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 November 22] Available at: https://urn.nsk.hr/urn:nbn:hr:159:941999

D. Puljić, "Površinski proteini Lactobacillus vrsta", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:941999

Metadata
TitlePovršinski proteini Lactobacillus vrsta
Title (english)Surface proteins of Lactobacillus species
AuthorDominika Puljić
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2017-07-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Kada je riječ o tehnološkim i zdravstvenim primjenama bakterija mliječne kiseline, sastav, struktura i komponente stanične stijenke, pogotovo površinski proteini, igraju glavne uloge. Stoga je cilj ovog rada bio dokazati prisutnost S-proteina i drugih površinskih proteina kod bakterijskih sojeva Lactobacillus helveticus M92, Lactobacillus brevis D6, Lactobacillus brevis ZG1, Lactobacillus brevis SF9B, Lactobacillus brevis SF15B i Lactobacillus plantarum D13. Provođenjem SDS-PAGE elektroforeze površinskih proteina dokazana je prisutnost Sproteina i SLAP (eng. Surface-Layer Associated Proteins). Potvrda prisutnosti S-proteina provedena je detekcijom gena koji kodiraju za S-proteine. Provedenom PCR reakcijom sa sojevima L. helveticus M92, L. brevis D6, L. brevis ZG1, L. brevis SF9B i L. brevis SF15B, s početnicama Sprot 1 i Sprot 2, a potom i DNA elektroforezom PCR produkata dobiveni su pozitivni signali za sve ispitivane Lactobacillus sojeve što ukazuje da su odabrane specifične početnice pogodne za detekciju gena koji kodiraju za površinske S-proteine.
Abstract (english)
When it comes to technological and health applications of lactic acid bacteria, the composition, structure and components of the cell wall, especially surface proteins, play the main roles. Therefore, the aim of this thesis was to prove the presence of S-layer and other surface proteins in bacterial strains of Lactobacillus helveticus M92, Lactobacillus brevis D6, Lactobacillus brevis ZG1, Lactobacillus brevis SF9B, Lactobacillus brevis SF15B and Lactobacillus plantarum D13. S-protein and SLAP (Surface-Layer Associated Proteins) were detected by SDS-PAGE electrophoresis of the surface proteins. Confirmation of the presence of S-protein was performed by detection of genes encoding S-proteins. PCR reaction with strains of L. helveticus M92, L. brevis D6, L. brevis ZG1, L. brevis SF9B and L. brevis SF15B with primers Sprot 1 and Sprot 2, and then DNA electrophoresis of PCR products gave the positive signals for all tested Lactobacillus strains which indicate that selected specific primers are suitable for the detection of genes encoding surface S-proteins.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:941999
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-07-20 11:34:29