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undergraduate thesis
Optimizacija lančane reakcije polimerazom za umnažanje mikrosatelitnih biljega divlje mačke Felis silvestris Schreber, 1777
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:816388
Jaša-Šangulin, Lucija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Jaša Šangulin, L. (2017). Optimizacija lančane reakcije polimerazom za umnažanje mikrosatelitnih biljega divlje mačke Felis silvestris Schreber, 1777 (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:816388

Jaša Šangulin, Lucija. "Optimizacija lančane reakcije polimerazom za umnažanje mikrosatelitnih biljega divlje mačke Felis silvestris Schreber, 1777." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:816388

Jaša Šangulin, Lucija. "Optimizacija lančane reakcije polimerazom za umnažanje mikrosatelitnih biljega divlje mačke Felis silvestris Schreber, 1777." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:816388

Jaša Šangulin, L. (2017). 'Optimizacija lančane reakcije polimerazom za umnažanje mikrosatelitnih biljega divlje mačke Felis silvestris Schreber, 1777', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:816388

Jaša Šangulin L. Optimizacija lančane reakcije polimerazom za umnažanje mikrosatelitnih biljega divlje mačke Felis silvestris Schreber, 1777 [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:816388

L. Jaša Šangulin, "Optimizacija lančane reakcije polimerazom za umnažanje mikrosatelitnih biljega divlje mačke Felis silvestris Schreber, 1777", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:816388

Metadata
TitleOptimizacija lančane reakcije polimerazom za umnažanje mikrosatelitnih biljega divlje mačke Felis silvestris Schreber, 1777
Title (english)Optimization of polymerase chain reaction for amplification of microsatellite markers of wildcat Felis silvestris Schreber, 1777
AuthorLucija Jaša-Šangulin
MentorAna Bielen (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2017-07-17, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Genomska ĉistoća populacije divlje maĉke u mnogim je europskim zemljama ugrožena zbog uĉestale hibridizacije s domaćom maĉkom. S ciljem utvrĊivanja stanja populacije u Hrvatskoj potrebno je provesti genotipizaciju divljih i domaćih maĉaka pomoću umnažanjem mikrosatelitnih biljega lanĉanom reakcijom polimerazom. U ovom je radu provedena optimizacija lanĉane reakcije polimerazom za umnažanje 12 odabranih mikrosatelitnih biljega divlje maĉke. UtvrĊena je optimalna koliĉina DNA kalupa od 10 ng i koncentracija oligonukleotidnih poĉetnica od 0.2 μM. Optimizirani su i uvjeti provoĊenja reakcije; temperatura vezanja poĉetnica od 57°C i vremena vezanja poĉetnica i produljenja lanaca od 90 sekundi. Konaĉno, opisani optimalni uvjeti prilagoĊeni su provedbi multipleks PCR-a radi uštede vremena i resursa. Rezultati ovog rada će u nastavku istraživanja poslužiti za umnažanje mikrosatelitnih biljega većeg broja divljih i domaćih maĉaka s ciljem odreĊivanja stupnja hibridizacije s domaćim maĉkama u Republici Hrvatskoj.
Abstract (english)
Genomic purity of European wildcat population is compromised by frequent hybridisation with domestic cats. Since the state Croatian wildcat population is unknow, it is necessary to carry out genotyping, i.e. amplify the microsatellite markers by polymerase chain reaction. In this work, optimization of PCR for amplification of 12 selected wildcat microsatellite markers was performed. An optimal amount of 10 ng DNA template and 0.2 μM of oligonucleotide primers was determined.Thermal cycling conditions were also optimized; optimal annealing temperature was 57°C and annealing and elongation time of 90 seconds. Finally, reaction conditions were further adjusted to perform multiplex PCR in order to save time and resources. Results of this work will be the basis of future amplification of microsatellite markers of wild and domestic cats in order to determine genomic purity of wildcat population in Croatia.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:816388
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-07-24 11:35:54