Sulfonamides are widely used in human and veterinary medicine for prevention and treatment of diseases. The consequence of excessive use of antibiotics for therapeutic purposes and as growth promoters animals leads to the emergence of resistant strains of bacteria. Antibiotic resistance represents a global public health problem due to the steadily rising number of resistant bacteria on the one hand, and the lack of antibiotics capable of efficiently treating infections caused by such multidrug resistant bacteria, on the other. For these reasons, it is necessary to control sulfonamides in bee products as one of the guidelines of veterinary public health in order to protect final consumers and prevent the spread of resistance. Specific legislation protects consumers from exposure to potentially harmful residues of veterinary medicines, in food of animal origin (Directive 96/23). The aim of this study was to deployment confirmatory methods for determination of sulfonamides in honey samples. For all samples the same procedure was used solid phase extraction (SPE) and the analytes are determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method has been validated, in accordance with EU Commission Decision 2002/657/EC by a computer program interval Plus and applied to the analysis of sulfonamides in honey samples collected from different parts of Croatian.