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undergraduate thesis
Lokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:787761
Keser, Vanessa
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Keser, V. (2016). Lokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:787761

Keser, Vanessa. "Lokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:787761

Keser, Vanessa. "Lokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:787761

Keser, V. (2016). 'Lokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:787761

Keser V. Lokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 December 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:787761

V. Keser, "Lokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:787761

Metadata
TitleLokalizacija proteina kontrolne točke diobenog vretena u mišjim spermatocitama
Title (english)Localization of spindle assembly checkpoint proteins in mouse spermatocytes
AuthorVanessa Keser
MentorAnamarija Štafa (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2016-06-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Tijekom mejoze, od velike je važnosti pravilna segregacija kromosoma, koja se ostvaruje zahvaljujući bipolarnom povezivanju niti diobenog vretena s kinetohorama kromosoma. Ukoliko bipolarno povezivanje nije ostvareno, kontrolna točka diobenog vretena (engl. Spindle Assembly Checkpoint, SAC) odgađa anafazu djelovanjem specifičnog kompleksa SAC, kojeg čine i proteini MAD1, MAD2, BUB1 i 3F3/2 fosfoepitop. Ako ova kontrolna točka nije funkcionalna, neostvarivanje bipolarne orijentacije diobenog vretena rezultira nepravilnom segregacijom kromosoma i promjenom broja kromosoma u stanicama kćeri. U ovome radu proteini MAD2 i 3F3/2 fosfoepitop, koji su dio kompleksa SAC, imunološki su obilježeni u svrhu određivanja njihove lokalizacije. Korišteni su transgeni Spo11β-only miševi, u čijim mejotičkim stanicama češće dolazi do nebipolarnog povezivanja kromosoma i mikrotubula nego kod miševa divljeg tipa. Utvrđeno je da proteini MAD2 i 3F3/2 fosfoepitop kolokaliziraju s proteinom SYCP3 u metafazi I.
Abstract (english)
During meiosis, appropriate segregation of chromosomes is of crucial importance, and it is achieved via bipolar orientation of kinetochore-microtubule attachment. Spindle assembly checkpoint (SAC), through a specific SAC complex consisting of MAD1, MAD2, BUB1 and 3F3/2 phosphoepitose and several other proteins, delays anaphase onset until all chromosomes are properly attached to the spindle. In case of malfunctioning SAC, cell will enter anaphase without proper kinetochore-microtubule orientation, and improper chromosomes segregation will occur, resulting in abnormal number of chromosomes in daughter cell. In this work proteins MAD2 and 3F3/2 phosphoepitope of the SAC complex were immunostained in order to determine their localization. Due to more frequent improper mitotic kinetochore-microtubule attachment, compared to the wild type, transgenic Spo11β-only mice were used. It was determined that both MAD2 and 3F3/2 phosphoepitope colocalize with SYCP3 in metaphase I.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:787761
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-04-24 13:16:19