Natural deep eutectic solvents (NADES) are new generation of environmentally friendly solvents with possible applications in various industrial fields. They are defined as a mixture of two or more components which can be connected to each other by hydrogen bonds, and which in a certain ratio have lower melting points than the individual components of the mixture. Because of the possibility of forming NADES from a wide range of compounds it is possible to adjust their properties to particular application. In this study, eight different NADES was prepared at different molar ratios (choline chloride: malic acid, proline: malic acid, choline chloride: proline: malic acid, betaine: malic acid, malic acid: glucose, malic acid: glucose: glycerol, choline chloride: citric acid, betaine: citric acid). Physico-chemical properties such as density, viscosity, refractive index, electrical conductivity and pH were measured and analyzed as a function of water content in the solvent (10, 30 and 50% v/v). Also, a biological characterization was conducted by exemination of their in vitro cytotoxicity on human cells HeLa and HEK293T and measurement of their antioxidant capacity with ORAC method. Because prepared NADES doesn't show cytotoxic effects toward treated cells, has antioxidant properties and the physico-chemical properties can be positively adjusted by increasing the water content, there is a possibility of their application in various technological processes.