Cultivation of amylolytic lactic acid bacterium Lactobacillus amylovorus DSM 20531T was conducted in modified MRS medium in which maltose (20.0 g L-1) was added instead of glucose. The cultivation of the bacterium in MRS medium with maltose (MRS-mal20) was carried out in a laboratory scale stirred tank bioreactor (working volume of 6.0 L) at constant temperature (40±0.2°C) and maintained pH value (pH of 5.5±0.2). Transport of maltose into bacterial cells started immediately after inoculation, transported maltose was hydrolysed and further degraded to lactic acid exclusively. Simultaneously with maltose utilization and lactic acid production bacterial biomass was synthesised (exponential growth) until t = 6.0 h of bioprocess, when bacterium entered stationary growth phase. After this time point utilization of maltose, bacterial biomass synthesis and lactic acid production were slowed down. Until the end of the bioprocess (t = 24.0 h) concentration of maltose decreased to 6.95 g L-1 and 11.34 g L-1 of lactic acid was produced. Resulting substrate to product conversion coefficient (YP/S) was only 0.87 g g-1. Strain L. amylovorus DSM 20531T can synthesise α-amylase and transport it to extracellular space. Isolation of the α-amylase was done by affinity chromatography. Supernatants of samples withdrawn at the end of exponentially and stationary growth phases were used in the isolation. Different α-amylase fractions were eluted and base-line separated by α-cyclodextrin solution. Fraction 1 (F1) consisted of several protein bands with molecular weight <30.0 kDa, as determined by SDSPAGE.