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undergraduate thesis
Obilježavanje i detekcija proteina Scw4 u soju SK1 kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:660559
Morduš, Josip
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Morduš, J. (2017). Obilježavanje i detekcija proteina Scw4 u soju SK1 kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:660559

Morduš, Josip. "Obilježavanje i detekcija proteina Scw4 u soju SK1 kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:660559

Morduš, Josip. "Obilježavanje i detekcija proteina Scw4 u soju SK1 kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:660559

Morduš, J. (2017). 'Obilježavanje i detekcija proteina Scw4 u soju SK1 kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:660559

Morduš J. Obilježavanje i detekcija proteina Scw4 u soju SK1 kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 December 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:660559

J. Morduš, "Obilježavanje i detekcija proteina Scw4 u soju SK1 kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:660559

Metadata
TitleObilježavanje i detekcija proteina Scw4 u soju SK1 kvasca Saccharomyces cerevisiae
Title (english)Tagging and detection of Scw4 protein in yeast Saccharomyces cerevisiae strain SK1
AuthorJosip Morduš
MentorIgor Stuparević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2017-08-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Za protein Scw4, koji se nalazi u staničnoj stijenci kvasca Saccharomyces cerevisiae, još uvijek nije poznato sudjeluje li u mejozi i sporulaciji te koja je njegova uloga u tim procesima. Taj protein nekovalentno i kovalentno je vezan za staničnu stijenku, pretpostavlja se da ima glukanaznu aktivnost i stoga vjerojatno sudjeluje u organizaciji glukana. Stanična stijenka, ekstracelularna je organela koja se sastoji od β-1,3 i β-1,6-glukana, hitina i manoproteina. Ulaskom u mejozu i sporulaciju dolazi do brojnih morfoloških i genetičkih promjena stanične stijenke, primjerice smanjenja ili povećanja ekspresije određenih proteina. Cilj ovog rada je utvrditi prisutnost proteina Scw4 tijekom mejoze i sporulacije. Dodatkom hemaglutininske (HA) oznake na C terminalni kraj proteina Scw4 omogućeno je praćenje ekspresije specifičnim anti-HA antitijelima nakon izolacije proteina. Obilježavanje i detekcija proteina uspješno su provedene, a Scw4 je u SDS-ekstraktu prisutan u mejozi i sporulaciji. Ovaj rad doprinosi razumijevanju uloge Scw4 proteina u staničnoj stijenci kvasca.
Abstract (english)
It is still not known if Scw4 protein in yeast Saccharomyces cerevisiae cell wall plays role in meiosis and sporulation. That protein is non-covalently and covalently attached to cell wall, and it's assumed that it has glucanase activity and probably participates in glucan organization. Cell wall is the extracellular organelle which consists of β-1,3 and β-1,6-glucan, chitin and mannoproteins. Meiosis and sporulation results in many morphological and genetical changes of cell wall, for example, decrease or increase of certain protein expression. The goal of this work is to determine presence of Scw4 during meiosis and sporulation. After protein isolation, it is enabled to track Scw4 expression with specific anti-HA antibodies by adding hemagglutinin (HA) tag to C-terminal residue of Scw4. Tagging and detection are successfully conducted and in SDS-extract Scw4 is present in meiosis and sporulation. This science work contributes in understanding of the role of Scw4 in cell wall.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:660559
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-08 12:18:07