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undergraduate thesis
Dodavanje hemaglutininske sekvence iza gena NCW2 u genom kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:155264
Bernat, Miroslava
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bernat, M. (2017). Dodavanje hemaglutininske sekvence iza gena NCW2 u genom kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:155264

Bernat, Miroslava. "Dodavanje hemaglutininske sekvence iza gena NCW2 u genom kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:155264

Bernat, Miroslava. "Dodavanje hemaglutininske sekvence iza gena NCW2 u genom kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:155264

Bernat, M. (2017). 'Dodavanje hemaglutininske sekvence iza gena NCW2 u genom kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:155264

Bernat M. Dodavanje hemaglutininske sekvence iza gena NCW2 u genom kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 June 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:155264

M. Bernat, "Dodavanje hemaglutininske sekvence iza gena NCW2 u genom kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:155264

Metadata
TitleDodavanje hemaglutininske sekvence iza gena NCW2 u genom kvasca Saccharomyces cerevisiae
Title (english)Adding hemagglutinin gene sequence behind NCW2 into the genome of Saccharomyces cerevisiae yeast
AuthorMiroslava Bernat
MentorIgor Stuparević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2017-08-30, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Stanična stijenka kvasca Saccharomyces cerevisiae kompleksna je izvanstanična organela čije su glavne komponente ß-1,3 i ß-1,6-glukan te manoproteini pričvršćeni za glukansku mrežu. Struktura stanične stijenke mijenja se u različitim fazama životnog ciklusa kvasca kao što su mitoza, mejoza i sporulacija. Za protein Ncw2 pokazano je da sudjeluje u organizaciji ß-glukanske mreže u staničnoj stijenci kvasca, ali nije istražena prisutnost u mejotičkim i stanicama koje sporuliraju. Za identifikaciju i detekciju proteina razvijene su različite molekularno genetičke metode, a jedna od njih je dodavanje oznake na kraj proteina. Cilj rada bio je dodati oznaku na protein kako bi se detektirala njegova prisutnost tijekom mejoze i sporulacije. Konstruiran je soj kvasca kojemu je hemaglutininska sekvenca (HA) dodana iza gena NCW2, što omogućuje zajedničku translaciju proteina Ncw2 i dodane oznake. Takav konstrukt je detektiran koristeći antitijela za hemaglutininsku oznaku. Obilježavanje proteina ovim načinom bilo je uspješno, te je pokazana njegova stabilna ekspresija u mejotičkim i sporulirajućim stanicama.
Abstract (english)
Saccharomyces cerevisiae cell wall is complex extracellular organelle whose major components are ß-1,3 i ß-1,6-glucan and mannoproteins attached to glucan network. It is known that cell wall structure changes during different phases of yeast life cycle such as mitosis, meiosis and sporulation. It has been shown that Ncw2 protein takes part in ß-glucan network reorganization however his presence during meiosis and sporulation still has not been determined. The goal of this work was to tag Ncw2 which will enable us to detect his presence in meiotic and sporulating cells. The construct was made in which at genome level hemagglutinin sequence (HA) is downstream of ORF allowing a translational fusion of protein of interest to the HA tag. This technique enables us to track Ncw2 using specific antibodies. We have confirmed that this system provides efficient tagging and stable expression. Finally, we found that Ncw2 is present in mitotic, meiotic and sporulating cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:155264
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-11 07:21:27