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master's thesis
Priprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:232952
Ivanjko, Natalia
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Ivanjko, N. (2016). Priprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:232952

Ivanjko, Natalia. "Priprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:232952

Ivanjko, Natalia. "Priprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:232952

Ivanjko, N. (2016). 'Priprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2023, https://urn.nsk.hr/urn:nbn:hr:159:232952

Ivanjko N. Priprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2023 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:232952

N. Ivanjko, "Priprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:232952

Metadata
TitlePriprava prirodnih eutektičnih otapala i njihova primjena u krioprotekciji stanične linije
Title (english)Preparation of natural eutectic solvents and their application in cryoprotection of cell lines
AuthorNatalia Ivanjko
MentorIgor Slivac (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2016-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Prirodna eutektična otapala (eng. Natural Deep Eutectic Solvents, NADES) su nova generacija ekološki prihvatljivih otapala sa mogućim primjenama u različitim industrijskim područjima. Definiraju se kao smjesa dviju ili više komponenata koje se međusobno mogu povezati vodikovim vezama te koje u određenom omjeru imaju niže talište nego pojedinačne komponente smjese. S obzirom na njihovu široku primjenu, u ovom radu ispitana je mogućnost njihove primjene kao krioprotektanata na staničnoj liniji HEK 293T. U ovom radu pripravljena su dva različita NADES-a u različitim molarnim odnosima (kolin klorid:glukoza, prolin:glukoza) te s različitim udjelima vode. Učinkovitost ovih NADES-a kao krioprotektanata uspoređena je sa standardnim krioprotektantima, glicerolom i dimetil sulfoksidom. Istraživanje je pokazalo da testirani spojevi nisu dostojna zamjena standardnim kriprotektantima.
Abstract (english)
Natural deep eutectic solvents (NADES) are new generation of environmentally friendly solvents with potential application in various industrial fields. They are defined as a mixture of two or more components connected to each other by hydrogen bonds. At a certain ratio of their building components, NADES can have lower melting points than the individual components of the mixture. Considering their widespread application, NADES are tested as cryoprotectants on cell line HEK 293T. Two different NADES are made for this experiment; they are consisted of proline/glucose and choline chloride/glucose in different molar ratio and with different percentage of water content. Efficiency of those NADES are compared with standard cryoprotectants, glycerol and dimethyl sulfoxide. The results showed that NADES cannot serve as an appropriate alternative to the standard cryoprotectants.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:232952
Study programmeTitle: Food Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-25 10:19:25