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undergraduate thesis
Učinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:040504
Madaras, Manda
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Madaras, M. (2017). Učinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:040504

Madaras, Manda. "Učinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:040504

Madaras, Manda. "Učinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:040504

Madaras, M. (2017). 'Učinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 19 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:040504

Madaras M. Učinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 December 19] Available at: https://urn.nsk.hr/urn:nbn:hr:159:040504

M. Madaras, "Učinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:040504

Metadata
TitleUčinak 5-hidroksimetil furfurala i furfurala na rast plijesni Mortierella isabellina i sintezu lipida
Title (english)The effect of 5-hydroxymethyl furfuryl and furfuryl on growth of fungus Mortierella isabellina and lipid synthesis
AuthorManda Madaras
MentorMirela Ivančić - Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2017-09-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Oleaginozni mikroorganizmi proizvode lipide koji se mogu koristiti kao zamjenska sirovina za proizvodnju biodizela umjesto biljnih ulja. Oleaginozna plijesan Mortierella isabellina nakuplja triacilglicerole kao stanične rezervne lipide čiji udjel u suhoj tvari biomase iznosi i do 80 %. Lignocelulozna biomasa izvor je jeftinih ugljikohidrata koji se mogu koristiti kao izvor ugljika za rast mikroorganizma i sintezu proizvoda (npr. bioetanola). Procesom predobrade poboljšava se enzimska razgradnja lignocelulozne sirovine s celulazama te se povećava prinos reducirajućih šećera nastalih hidrolizom. Međutim tijekom ovog procesa nastaje niz nusproizvoda iz lignocelulozne sirovine koji inhibiraju radni mikroorganizam i celulolitičke enzime. U ovom radu istražen je učinak furfurala i 5-hidroksimetil furfurala (HMF) na rast i proizvodnju lipida s plijesni M. isabellina. Rezultati pokazuju da furfural jače inhibira rast i sintezu lipida od HMF-a. Rast plijesni i sinteza lipida je potpuno inhibirana pri koncentraciji furfurala većoj od 0,6 g/L i HMF-a većoj od 2 g/L.
Abstract (english)
Lipids produced by oleaginous microorganisms can be used as alternative feedstock for production of biodiesel instead of plant oils. Oleaginous fungus Mortierella isabellina has the ability to accumulate triacylglycerols, as cellular storage lipids, up to 80% of the biomass. Lignocellulosic biomass is source of cheap carbohydrates that can be used as carbon source for the microbial growth and product synthesis (e.g. bioethanol production). The pretreatment process improves enzymatic digestibility of lignocellulosic biomass by cellulases and enhanced the yield of reducing sugars by hydrolysis. However, during this process many lignocellulose-derived by-products are formed that inhibit microorganism and cellulolic enzymes. The influence of furfural and 5-hydroxymethyl furfural (HMF) on the growth and lipid production by fungus M. isabellina was investigated. The results indicate that furfural inhibits the growth and lipid synthesis markedly more than HMF. The growth and lipid accumulation of fungus was completely inhibited at concentration of furfural and HMF higher than 0,6 g/L and 2 g/L, respectively.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:040504
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-18 08:18:18