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undergraduate thesis
Učinak reciklacije lignocelulolitičkih enzima na proizvodnju lipida s kvascem Trichosporon oleaginosus uzgojenim na hidrolizatu kukuruznog oklaska
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:224478
Gruičić, Ana-Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Gruičić, A. M. (2017). Učinak reciklacije lignocelulolitičkih enzima na proizvodnju lipida s kvascem Trichosporon oleaginosus uzgojenim na hidrolizatu kukuruznog oklaska (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:224478

Gruičić, Ana Marija. "Učinak reciklacije lignocelulolitičkih enzima na proizvodnju lipida s kvascem Trichosporon oleaginosus uzgojenim na hidrolizatu kukuruznog oklaska." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:224478

Gruičić, Ana Marija. "Učinak reciklacije lignocelulolitičkih enzima na proizvodnju lipida s kvascem Trichosporon oleaginosus uzgojenim na hidrolizatu kukuruznog oklaska." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:224478

Gruičić, A. M. (2017). 'Učinak reciklacije lignocelulolitičkih enzima na proizvodnju lipida s kvascem Trichosporon oleaginosus uzgojenim na hidrolizatu kukuruznog oklaska', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 11 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:224478

Gruičić AM. Učinak reciklacije lignocelulolitičkih enzima na proizvodnju lipida s kvascem Trichosporon oleaginosus uzgojenim na hidrolizatu kukuruznog oklaska [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 July 11] Available at: https://urn.nsk.hr/urn:nbn:hr:159:224478

A. M. Gruičić, "Učinak reciklacije lignocelulolitičkih enzima na proizvodnju lipida s kvascem Trichosporon oleaginosus uzgojenim na hidrolizatu kukuruznog oklaska", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:224478

Metadata
TitleUčinak reciklacije lignocelulolitičkih enzima na proizvodnju lipida s kvascem Trichosporon oleaginosus uzgojenim na hidrolizatu kukuruznog oklaska
Title (english)The effect of lignocellulosic enzymes recycling on lipid production by yeast Trichosporon oleaginosus grown on corn cob hydrolysate
AuthorAna-Marija Gruičić
MentorMirela Ivančić Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2017-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Mikrobni lipidi, proizvedeni uz pomoć oleaginoznih mikroorganizama na lignoceluloznim supstratima, obećavajuća su alternativa nafti kao sirovina za proizvodnju dizela. Oleaginozni kvasac Trichosporon oleaginosus može nakupiti i do 80% lipida u biomasi prilikom rasta u uvjetima limitacije dušikom. Mikrobna proizvodnja lipida uključuje hidrolizu lignocelulozne sirovine do fermentabilnih šećera, koji se koriste za mikrobni rast i sintezu lipida. U ovom radu prikazan je novi proces odvojene hidrolize i proizvodnje lipida uz reciklaciju enzima, koji je proveden u četiri ponovljena ciklusa. Nehidrolizirani, teško razgradivi čvrsti ostatak s adsorbiranim celulazama, izdvojen je i nanovo korišten u narednim ciklusima enzimske hidrolize. Dobiveni rezultati pokazali su da se reciklacijom nehidrolizirane biomase u četiri ciklusa smanjuje dodana količina enzima do 30% njegove optimalne količine, bez značajnijeg utjecaja na učinkovitost hidrolize lignocelulozne sirovine i produktivnost nakupljanja lipida. Prosječna vrijednost koncentracije biomase iznosila je 23,23 gL-1, a udio lipida u biomasi 47,58 %.
Abstract (english)
Microbial lipids produced from lignocellulosic biomass by oleaginous microorganisms are promising alternative feedstock to petroleum in biodiesel production. Oleaginous yeast Trichosporon oleaginosus has the ability to accumulate lipids up to 80% of the biomass under nitrogen-limited growth conditions. Microbial lipid production involves hydrolysis of lignocellulosic biomass into fermentable sugars, which are used for microbial growth and lipid synthesis. Here we report a novel process of the separate hydrolysis and lipid production with enzyme recycling conducted in four repeated cycles. Unhydrolysed recalcitrant solids with adsorbed cellulases were separated and recycled to the subsequent cycles of enzymatic hydrolysis. Obtained results indicated that enzyme recycling with the unhydrolysed solids reduced the enzyme loading up to 30% of the optimal enzyme loading without significant effect on efficiency of lignocellulose hydrolysis and lipid productivity. Average value of biomass concentration and lipid content was 23,23 gL-1 and 47,58 %, respectively.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:224478
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-09-20 07:26:59