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undergraduate thesis
Kompatibilnost deset često korištenih fluorescentnih proteina s mikroskopom EVOS Floid
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:678393
Stošić, Irin Antun
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Stošić, I. A. (2017). Kompatibilnost deset često korištenih fluorescentnih proteina s mikroskopom EVOS Floid (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:678393

Stošić, Irin Antun. "Kompatibilnost deset često korištenih fluorescentnih proteina s mikroskopom EVOS Floid." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:678393

Stošić, Irin Antun. "Kompatibilnost deset često korištenih fluorescentnih proteina s mikroskopom EVOS Floid." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:678393

Stošić, I. A. (2017). 'Kompatibilnost deset često korištenih fluorescentnih proteina s mikroskopom EVOS Floid', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 03 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:678393

Stošić IA. Kompatibilnost deset često korištenih fluorescentnih proteina s mikroskopom EVOS Floid [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 December 03] Available at: https://urn.nsk.hr/urn:nbn:hr:159:678393

I. A. Stošić, "Kompatibilnost deset često korištenih fluorescentnih proteina s mikroskopom EVOS Floid", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:678393

Metadata
TitleKompatibilnost deset često korištenih fluorescentnih proteina s mikroskopom EVOS Floid
Title (english)Compatibility of ten frequently used fluorescent proteins with EVOS Floid microscope
AuthorIrin Antun Stošić
MentorIvan Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2017-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Cilj ovog rada bio je istražiti kompatibilnost deset često korištenih fluorescentnih proteina s fluorescencijskim mikroskopom EVOS Floid, odnosno koji su od 10 proteina (mTagBFP2, mTFP1, mEGFP, mCitrine, tdTomato, mTagRFP, mCherry, mKate, mPlum i E2Crimson) vidljivi te mogu li se međusobno razlikovati ovim mikroskopom koji kao izvor svjetlosti koristi plave, crvene i zelene svjetleće diode. Plazmidi koji nose gene za fluorescentne proteine nabavljeni su u bakteriji E. coli, soj DH5α, izolirani su iz tog soja i njima je transformiran soj E. coli NEB Stable prikladniji za ekspresiju gena pod kontrolom promotora operona araBAD. Struktura plazmida potvrđena je restrikcijskom analizom, a mikroskopiranjem stanica E. coli s eksprimiranim fluorescentnim proteinima utvrđeno je da su EVOS Floid mikroskopom vidljivi svi proteini osim mTagRFP te da je u istom uzorku međusobno moguće razlikovati do pet različitih fluorescentnih proteina.
Abstract (english)
The aim of this paper was to determine the compatibility of ten commonly used fluorescent proteins with the EVOS Floid fluorescence microscope. More specifically to determine which of the ten proteins (mTagBFP2, mTFP1, mEGFP, mCitrine, tdTomato, mTagRFP, mCherry, mKate, mPlum, and E2Crimson) are visible, and the way they differ from one another under this microscope that uses blue, red and green light emitting diodes as a light source. Plasmids carrying fluorescent protein genes were obtained in the DH5α E. coli strain, then isolated from this strain and the E. coli NEB Stable strain was transformed with them. The NEB Stable strain is more suitable for gene expression controlled by the araBAD promoter. The structure of plasmids was confirmed with restriction analysis. All proteins except for mTagRFP are visible with EVOS Floid microscope, and it is possible to distinguish between up to five fluorescent proteins in the same sample.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:678393
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-09-20 08:07:51