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undergraduate thesis
Ekspresija rekombinantne ksiloza reduktaze na površini stanice kvasca S. cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:736425
Švec, Danijel
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Švec, D. (2017). Ekspresija rekombinantne ksiloza reduktaze na površini stanice kvasca S. cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:736425

Švec, Danijel. "Ekspresija rekombinantne ksiloza reduktaze na površini stanice kvasca S. cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:736425

Švec, Danijel. "Ekspresija rekombinantne ksiloza reduktaze na površini stanice kvasca S. cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:736425

Švec, D. (2017). 'Ekspresija rekombinantne ksiloza reduktaze na površini stanice kvasca S. cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 20 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:736425

Švec D. Ekspresija rekombinantne ksiloza reduktaze na površini stanice kvasca S. cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 November 20] Available at: https://urn.nsk.hr/urn:nbn:hr:159:736425

D. Švec, "Ekspresija rekombinantne ksiloza reduktaze na površini stanice kvasca S. cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:736425

Metadata
TitleEkspresija rekombinantne ksiloza reduktaze na površini stanice kvasca S. cerevisiae
Title (english)Expression of the recombinant xylose reductase on the surface of yeast cell S. cerevisiae
AuthorDanijel Švec
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2017-09-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Saccharomyces cerevisiae jedan je od najčešće korištenih mikroorganizama u biotehnološkoj proizvodnji, pa se tako koristi i u proizvodnji ksilitola, prirodnog zaslađivača s velikim prehrambenim prednostima. Enzim odgovoran za mikrobiološku proizvodnju ksilitola, ksiloza reduktaza, katalizira reakciju redukcije ksiloze u ksilitol. Ksiloza reduktaza prirodno je lokalizirana u citoplazmi stanice kvasca što znači da izolacija finalnog proizvoda uključuje razbijanje stanice i više koraka pročišćavanja. Zbog relativno malog prinosa i kompleksnog pročišćavanja ksilitol na tržištu ima visoku cijenu. Tema ovog rada jest ekspresija i imobilizacija heterologne rekombinantne ksiloza reduktaze na površini stanice kvasca čime bi se izolacija proizvoda olakšala, a cijena konačnog proizvoda snizila. U tu je svrhu iskorišten protein Pir4, protein stanične stijenke kvasca, s kojim je ksiloza reduktaza fuzionirana i preko kojeg je vezana na staničnu stijenku. U radu je ispitana uspješnost ekspresije i ugradnje u stijenku te aktivnost tako imobiliziranog rekombinantnog enzima.
Abstract (english)
Saccharomyces cerevisiae is one of the most used microorganisms in biotechnological production, so it's also used in production of xylitol, natural sweetener with many nutritional advantages. Enzyme responsible for microbiological production of xylitol, xylose reductase, catalyses the reduction reaction of xylose to xylitol. Xylose reductase is naturally localized in yeast cell cytoplasm meaning that the isolation of the final product includes breaking the yeast cell and several steps of purification. Because of low yield and complexity of purification, xylitol has high price on the market. Theme of this thesis is expression and immobilization of heterologous recombinant xylose reductase on the surface of yeast cell meaning that the isolation of product would be easier and the price lower. For that purpose yeast cell wall protein Pir4is fused with xylose reductase serving as anchor through which xylose reductase binds to the yeast cell wall. In this thesis, the efficacy of expression and incorporation of the immobilized recombinant enzyme to the cell wall is examined, as well as its activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:736425
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-09-20 09:21:11