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master's thesis
Utjecaj mikotoksina na parametre rasta bakterija mliječne kiseline, potencijalnih starter kultura za proizvodnju fermentiranih namirnica
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:503234
Prtenjača, Jure
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for General Microbiology and Food Microbiology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Prtenjača, J. (2017). Utjecaj mikotoksina na parametre rasta bakterija mliječne kiseline, potencijalnih starter kultura za proizvodnju fermentiranih namirnica (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:503234

Prtenjača, Jure. "Utjecaj mikotoksina na parametre rasta bakterija mliječne kiseline, potencijalnih starter kultura za proizvodnju fermentiranih namirnica." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:503234

Prtenjača, Jure. "Utjecaj mikotoksina na parametre rasta bakterija mliječne kiseline, potencijalnih starter kultura za proizvodnju fermentiranih namirnica." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:503234

Prtenjača, J. (2017). 'Utjecaj mikotoksina na parametre rasta bakterija mliječne kiseline, potencijalnih starter kultura za proizvodnju fermentiranih namirnica', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 03 October 2024, https://urn.nsk.hr/urn:nbn:hr:159:503234

Prtenjača J. Utjecaj mikotoksina na parametre rasta bakterija mliječne kiseline, potencijalnih starter kultura za proizvodnju fermentiranih namirnica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 October 03] Available at: https://urn.nsk.hr/urn:nbn:hr:159:503234

J. Prtenjača, "Utjecaj mikotoksina na parametre rasta bakterija mliječne kiseline, potencijalnih starter kultura za proizvodnju fermentiranih namirnica", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:503234

Metadata
TitleUtjecaj mikotoksina na parametre rasta bakterija mliječne kiseline, potencijalnih starter kultura za proizvodnju fermentiranih namirnica
Title (english)Effect of mycotoxins on parameters of lactic acid bacteria, potential starter cultures for the production of fermented foodstuffs
AuthorJure Prtenjača
MentorKsenija Markov (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for General Microbiology and Food Microbiology)
Zagreb
Defense date and country2017-09-28, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Abstract
Prema procjeni FAO-a, 25% hrane proizvedeno u svijetu kontaminirano je mikotoksinima. Zbog toksičnog učinka, ne samo na ljude, životinje i biljke već i mikroorganizme, postoji zabrinutost zbog ulaska mikotoksina u prehrambeni lanac čovjeka, tzv. „carry over“ efektom. Cilj ovog rada bio je istražiti utjecaj aflatoksina B1 (AFB1) i zearalenona (ZEA) na rast i morfološke osobine odabranih sojeva bakterija mliječne kiseline Lactobacillus plantarum K1, Lactobacillus plantarum B, Leuconostoc mesenteroides i Pediococcus pentosaceus, potencijalnih starter kultura za proizvodnju fermentiranih namirnica. Mikrobni rast ovisi o uvjetima okoline u kojoj se mikroorganizmi nalaze kao i izloženosti kemijskim agensima, a toksično djelovanje mikotoksina najčešće se očituje kroz inhibiciju rasta. Rast bakterija mliječne kiseline (BMK) ispitivan je u prisutnosti AFB1 i ZEA različitih koncentracija tijekom 24 satnog uzgoja u MRS podlozi, a izražen je brojanjem poraslih kolonija na čvrstoj hranjivoj podlozi u obliku log10 CFU/mL. Metoda mikrometrije korištena je za određivanje veličine stanica, kao morfološke osobine. Iz dobivenih rezultata uočeno je da istraživani mikotoksini u različitim koncentracijama imaju utjecaj na veličinu, broj živih stanica, ali ne i na konačan broj ispitivanih stanica BMK.
Abstract (english)
According to FAO estimates, 25% of food produced in the world is contaminated with mycotoxins. Because of the toxic effect not only on humans, animals and plants, but also on microorganisms, there is concern about the introduction of mycotoxins into the human food chain, by so-called „carry over“ effect. The aim of this paper was to investigate the effects of aflatoxin B1 (AFB1) and zearalenone (ZEA) on growth and morphological properties of selected strains of lactic acid bacteria Lactobacillus plantarum K1, Lactobacillus plantarum B, Leuconostoc mesenteroides and Pediococcus pentosaceus, potential starter cultures for fermented foods. Microbial growth depends on the conditions of the environment in wich microorganisms are found as well as exposure to chemical agents, and the toxicity of mycotoxins is most often manifested through growth inhibition. The growth of lactic acid bacteria (LAB) was investigated in the presence of AFB1 and ZEA at various concentrations during 24 hours of cultivation in the MRS broth and expressed by counting the growing colonies on a solid nutrient medium in the form of log10 CFU/mL. The micrometric method was used to determine the cell size as a morphological trait. From the obtained results it is observed that mycotoxins at different concentrations have an effect on size, number of live cells but not on the final number of LAB cells.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:503234
Study programmeTitle: Food Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka prehrambenog inženjerstva (magistar/magistra inženjer/inženjerka prehrambenog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-07 10:20:45