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master's thesis
Usporedba citotoksičnih učinaka PCB-153 određenih različitim in vitro metodama
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:496113
Zubić, Jelena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Toxicology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Zubić, J. (2017). Usporedba citotoksičnih učinaka PCB-153 određenih različitim in vitro metodama (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:496113

Zubić, Jelena. "Usporedba citotoksičnih učinaka PCB-153 određenih različitim in vitro metodama." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:496113

Zubić, Jelena. "Usporedba citotoksičnih učinaka PCB-153 određenih različitim in vitro metodama." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:496113

Zubić, J. (2017). 'Usporedba citotoksičnih učinaka PCB-153 određenih različitim in vitro metodama', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 10 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:496113

Zubić J. Usporedba citotoksičnih učinaka PCB-153 određenih različitim in vitro metodama [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 July 10] Available at: https://urn.nsk.hr/urn:nbn:hr:159:496113

J. Zubić, "Usporedba citotoksičnih učinaka PCB-153 određenih različitim in vitro metodama", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:496113

Metadata
TitleUsporedba citotoksičnih učinaka PCB-153 određenih različitim in vitro metodama
Title (english)Comparison of PCB-153 cytotoxic effects determined by different in vitro methods
AuthorJelena Zubić
MentorIvana Kmetič (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Toxicology)
Zagreb
Defense date and country2017-06-18, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U ovom radu ispitan je učinak neplanarnog PCB-153 (10-100 μM) na proliferaciju CHO (Chinese Hamster Ovary)-K1 stanica metodama Neutral Red, Kenacid Blue, MTT i Trypan Blue nakon 6 i 48 h tretmana. Metodom protočne citometrije određen je postotak živih i mrtvih stanica, kao i postotak stanica u apoptozi. Nakon 6 h tretmana značajan inhibicijski učinak CHO-K1 stanica određen je kod tri provedene metode pri koncentracijama PCB-153 od 75 i 100 μM, izuzev kod metode Kenacid Blue. Nakon 48 h PCB-153 je značajno inhibirao staničnu proliferaciju pri najvišim koncentracijama (75 i 100 μM) kod sve četiri provedene metode. Metodom protočne citometrije utvrđeno je da se proporcionalno koncentraciji PCB-153 povećava broj stanica u apoptozi nakon 6 h tretmana (12,18 % - 28,15 %), a nakon 48 h broj mrtvih stanica (5,75 % - 33,43 %).
Abstract (english)
The aim of this study was to examine the cytotoxic effects of the non-planar PCB-153 (10-100 μM) by monitoring CHO (Chinese Hamster Ovary)-K1 cell proliferation using Neutral Red, Kenacid Blue, MTT and Trypan Blue methods after 6 and 48 hours of treatment. Flow cytometry was used to distinguish live, dead and apoptotic cells. Statistically significant inhibition was determined with 75 and 100 μM PCB after 6 hours with three methods used, except Kenacid Blue. Also, statistically significant decrease of cell proliferation was detected with the higest PCB-153 concentrations (75 and 100 μM) after 48 hours with all four applied methods. The flow cytometry analysis revealed that increase in PCB-153 concentration proportionaly increased the number of apoptotic cells after 6 hours (12,18 % - 28,15 %) and number of dead cells (5,75 % - 33,43 %) after 48 hours of treatment.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:496113
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-08 09:27:57