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master's thesis
Utjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:609397
Vukšić, Martina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Vukšić, M. (2017). Utjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:609397

Vukšić, Martina. "Utjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:609397

Vukšić, Martina. "Utjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:609397

Vukšić, M. (2017). 'Utjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 30 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:609397

Vukšić M. Utjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 November 30] Available at: https://urn.nsk.hr/urn:nbn:hr:159:609397

M. Vukšić, "Utjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:609397

Metadata
TitleUtjecaj eutektičkih otapala na lipazom kataliziranu hidrolizu (R,S)-1-feniletil-acetata
Title (english)The effect of deep eutectic solvents on lipase catalyzed hydrolysis of (R,S)-1-phenylethyl-acetate
AuthorMartina Vukšić
MentorMarina Cvjetko Bubalo (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2017-09-29, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U svrhu uspostavljanja visokoučinkovitih procesa proizvodnje industrijski važnih kemikalija, u posljednjih nekoliko godina eutektička otapala intenzivno se proučavaju kao zelena zamjena za škodljiva otapala u procesima organske sinteze i biokatalize. U ovom radu ispitana je mogućnost primjene lipaze B kao biokatalizatora u hidrolizi (R,S)-1-feniletil-acetata u kolinijevim eutektičkim otapalima. Uspoređujući sve ispitane parametre (η, VP, ee i VE), zaključuje se da su eutektička otapala superiornija za lipazom kataliziranu hidrolizu od konvencionalnih organskih otapala, pri čemu se otapalo ChGlc s 50 % vode pokazalo kao najbolje. U istoimenom otapalu kao i u puferu, ispitana je termička stabilnost lipaze kroz vremenski period od 72h u temperaturnom rasponu od 20-80 °C. Pri tome je ustanovljeno da u tercijarnim mješavinama DES-a ChGlc nakon 72 h inkubacije, najveći postotak inicijalne aktivnosti lipaza zadržava pri temperaturi od 40 °C. Osim toga, ustanovljeno je da lipaza pokazuje veću termičku stabilnost u eutektičkim otapalima nego u puferu.
Abstract (english)
For the purpose of establishing new sustainable processes for the production of industrially important compounds, in recent years deep eutectic solvents have been intensively studied as a possible replacement for organic solvents for biocatalytic and organic reactions. Within this work, the hydrolysis of (R,S)-1-phenylethyl acetate within cholinium-based deep eutectic solvents was studied. Comparing all the tested parameters (η, VP, ee and VE), it is clear that deep eutectic solvents are superior to conventional organic solvents for lipase catalyzed hydrolysis, where solvent ChGlc with 50 % of water was found to be the best between all used. Thermal stability of lipase at different temperatures through a certain period of time, was also examined. It was established that after 72 hours of incubation in tertiary DES mixtures, lipase retains its higest activity at 40 °C. Thermal stability of lipase was proven to be higher in deep eutectic solvents than in buffer.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:609397
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-08 10:08:56