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master's thesis
Procjena učinka nesteroidnih protuupalnih lijekova u kulturama životinjskih stanica
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:138781
Jakopec, Marta
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Jakopec, M. (2017). Procjena učinka nesteroidnih protuupalnih lijekova u kulturama životinjskih stanica (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:138781

Jakopec, Marta. "Procjena učinka nesteroidnih protuupalnih lijekova u kulturama životinjskih stanica." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:138781

Jakopec, Marta. "Procjena učinka nesteroidnih protuupalnih lijekova u kulturama životinjskih stanica." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:138781

Jakopec, M. (2017). 'Procjena učinka nesteroidnih protuupalnih lijekova u kulturama životinjskih stanica', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 05 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:138781

Jakopec M. Procjena učinka nesteroidnih protuupalnih lijekova u kulturama životinjskih stanica [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 August 05] Available at: https://urn.nsk.hr/urn:nbn:hr:159:138781

M. Jakopec, "Procjena učinka nesteroidnih protuupalnih lijekova u kulturama životinjskih stanica", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:138781

Metadata
TitleProcjena učinka nesteroidnih protuupalnih lijekova u kulturama životinjskih stanica
Title (english)Effects estimation of nonsteroidal anti-inflammatory drugs in animal cell cultures
AuthorMarta Jakopec
MentorKristina Radošević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2017-07-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Nesteroidni protuupalni lijekovi (NSAID, eng. nonsteroidal anti-inflammatory drug) intenzivno se istraţuju posljednjih desetljeća obzirom na njihov antitumorski potencijal te smanjenje rizika za razvoj i napredak malignih bolesti njihovom primjenom. Cilj ovog rada je ispitati in vitro učinak 25 različitih NSAID-ova na humanim staničnim linijama te antitumorski potencijal i mehanizam djelovanja onih s najizraţenijim citotoksičnim učinkom. Ispitani NSAID-i imaju potencijal kao antitumorski lijekovi obzirom na njihov jači antiproliferativni učinak na tumorske stanice, MCF-7, HepG2 i HeLa, nego na HEK293T stanice porijeklom iz zdravog tkiva, za koje su izračunate najveće vrijednosti IC50. Mogući mehanizmi djelovanja NSAID-a uključuju indukciju apoptoze, koja je značajnija kod tumorskih stanica nego kod normalnih stanica, te indukciju zastoja staničnog ciklusa u G0/G1 fazi odgovornu za antiproliferacijski učinak u tumorskim staničnim linijama.
Abstract (english)
Nonsteroidal anti-inflammatory drugs (NSAID) have been intensively investigated in the last decades in view of their anticancer potential and reducing the risk of developing and progressing malignant diseases by their application. The aim of this paper is to examine the in vitro effect of 25 different NSAIDs on human cell lines and the anticancer potential and mechanism of action of those with the most potent cytotoxic effect. Studied NSAIDs have potential as antitumor drugs due to their stronger antiproliferative effect on tumor cells, MCF-7, HepG2 and HeLa than on HEK293T cells derived from healthy tissue that have the highest calculated IC50 values. Possible mechanisms of NSAIDs action include induction of apoptosis, which is more significant in tumor cells than in normal cells, and induction of cell cycle arrest in the G0/G1 stage responsible for the antiproliferative effect in tumor cell lines.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:138781
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-09 10:16:08