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master's thesis
Proizvodnja bioetanola na blago kiselinskim hidrolizatima pšenične slame dobivenim pomoću sumporne kiseline u različitim mjerilima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:959653
Grubišić, Marina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Grubišić, M. (2017). Proizvodnja bioetanola na blago kiselinskim hidrolizatima pšenične slame dobivenim pomoću sumporne kiseline u različitim mjerilima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:959653

Grubišić, Marina. "Proizvodnja bioetanola na blago kiselinskim hidrolizatima pšenične slame dobivenim pomoću sumporne kiseline u različitim mjerilima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:959653

Grubišić, Marina. "Proizvodnja bioetanola na blago kiselinskim hidrolizatima pšenične slame dobivenim pomoću sumporne kiseline u različitim mjerilima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:959653

Grubišić, M. (2017). 'Proizvodnja bioetanola na blago kiselinskim hidrolizatima pšenične slame dobivenim pomoću sumporne kiseline u različitim mjerilima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:959653

Grubišić M. Proizvodnja bioetanola na blago kiselinskim hidrolizatima pšenične slame dobivenim pomoću sumporne kiseline u različitim mjerilima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:959653

M. Grubišić, "Proizvodnja bioetanola na blago kiselinskim hidrolizatima pšenične slame dobivenim pomoću sumporne kiseline u različitim mjerilima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:959653

Metadata
TitleProizvodnja bioetanola na blago kiselinskim hidrolizatima pšenične slame dobivenim pomoću sumporne kiseline u različitim mjerilima
Title (english)Production of bioethanol on the mild acid wheat straw hydrolysates obtained by sulfuric acid in various scales
AuthorMarina Grubišić
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2017-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Kako bi se ispitao utjecaj temperature na uspješnost procesa predobrade pšenične slame s 0,5 % sumpornom kiselinom u visokotlačnom reaktoru, procesi predobrade provedeni su na temperaturama od 150 oC, 160 oC, 170 oC, 180 oC, 190 oC i 200 oC uz konstantno vrijeme zadržavanja (tz) od 5 minuta. Najviša koncentracija ksiloze (13,0154 g L-1) ostvarena je pri temperaturi od 160 oC, a glukoze (4,7257 g L-1) na temperaturi od 200 oC. Kako bi se ispitao učinak vremena zadržavanja na uspješnost procesa, primjenjena su vremena zadržavanja od 1 i 10 minuta na tri temperature: 160, 180 i 200 oC. Najveći maseni udio glukana uočen je predobradom na 200 oC i tz = 10 min i iznosi 30,42 %, a ksilana na temperaturi od 160 oC i tz =5 min i iznosi 68,64 %. Analizom sastava hidrolizata odabrano je 5 različitih hidrolizata za usporedbu rasta i proizvodnje etanola u Erlenmeyer tikvicama pomoću dva soja kvasca S. cerevisiae: (1) divlji soj i soj Y9. Najveća uspješnost procesa proizvodnje etanola zabilježena je kod uzgoja soja Y9 na hidrolizatu dobivenom predobradom na temperaturi od 180 oC i vremenu zadržavanja od 10 minuta pri čemu je ostvarena produktivnost od 0,045 g L-1 h-1 i efikasnost od 43,25 %. Na istom hidrolizatu proveden je uzgoj kvasca soj Y9 i u poluindustrijskom mjerilu tj. horizontalnom rotirajućem cijevnom bioreaktoru pri čemu je dobivena produktivnost od 0,143 g L-1 h-1 i efikasnost od 64,01 %.
Abstract (english)
In order to investigate the effect of temperature on the efficancy of wheat straw pretretmant with 0,5 % sulfuric acid in high-pressure reactor, processes were conducted on different temperatures: 150 oC, 160 oC, 170 oC, 180 oC, 190 oC and 200 oC, with a constant residence time of 5 minutes. The highest xylose concentration (13,0154 g L-1) was achieved in pretretmant carried out at temperature of 160 oC and the highest glucose concentration (4,7257 g L-1) at temperature of 200 oC respectively. To investigate the effect of residence time on the efficancy of procces, residance time of 1 and 10 minutes were were also used at three temperatures: 160, 180 and 200 oC. The highest mass fraction of xylan (68,64 %) was achieved at temperature of 160 oC and residence time of 5 minutes. The highest mass fraction of glucan was 30,42 %, and it was observed at temperature of 200 oC and residence time of 10 minutes. Based on the composition of acid hydrolysates of wheat straw, five hydrolysates were selected for further research with two strains of S. cerevisiae: wild-type and type Y9. The most successful ethanol production process was observed by yeast strain Y9 on the broth containing wheat straw hydrolysates obtained during pretretmant at 180 oC and residence time of 10 minutes. In this bioprocess productivity of 0,045 g L-1 h-1 together with efficiency of 43,25 % were observed. During anaerobic cultivation of S. cerevisiae strain Y9 in the horizontal rotating tubular bioreactor on the same hydrolysate bioprocess productivity reached value of 0,143 g L-1 and efficiency of 64,01 % respectively.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:959653
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-09 10:58:55