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master's thesis
Potential of soya substrates for lactic acid fermentation with addition of Lactobacillus plantarum
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:052442
Dujmić, Elena
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Dujmić, E. (2017). Potential of soya substrates for lactic acid fermentation with addition of Lactobacillus plantarum (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:052442

Dujmić, Elena. "Potential of soya substrates for lactic acid fermentation with addition of Lactobacillus plantarum." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:052442

Dujmić, Elena. "Potential of soya substrates for lactic acid fermentation with addition of Lactobacillus plantarum." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:052442

Dujmić, E. (2017). 'Potential of soya substrates for lactic acid fermentation with addition of Lactobacillus plantarum', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:052442

Dujmić E. Potential of soya substrates for lactic acid fermentation with addition of Lactobacillus plantarum [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2025 February 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:052442

E. Dujmić, "Potential of soya substrates for lactic acid fermentation with addition of Lactobacillus plantarum", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:052442

Metadata
TitlePotential of soya substrates for lactic acid fermentation with addition of Lactobacillus plantarum
Title (croatian)Pogodnost sojinih supstrata za mliječno kiselu fermentaciju uz dodatak bakterije Lactobacillus plantarum
AuthorElena Dujmić
MentorLidija Šver (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2017-09-27, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
In this work, growth dynamics of Fresco DVS 1010 culture and human derived isolate Lactobacillus plantarum in milk- water- or lactose-free milk-based soya substrates with sucrose or flavouring compounds (chocolate or caramel) were evaluated. Static anaerobic fermentation was carried out for 8 hours at 37 ± 0.5°C (5% CO2), afterward with storage period for 3 weeks at 6 ± 0.5°C. Although milk is a typical growth medium for lactic acid bacteria, presumable viable counts of Fresco culture reached levels 109 CFU mL-1 after 8 h. Potentially probiotic isolate L. plantarum added after the fermentation process maintained its viability during cold storage above the limit 106 CFU mL-1. Furthermore, the effect of temperature (8-40°C) on the growth dynamics of L. plantarum in a model environment of UHT lactose-free milk (1.5% fat) was investigated. Final densities of studied isolate reached counts 107 CFU mL-1 in stationary phase at all studied temperatures (except for marginal 8°C). During the growth and multiplication of L. plantarum, as a result of metabolic activity, the decrease of pH from initial values about 0.17-1.16 units was recorded. Experimentally, it was found that optimal temperature was close to 34°C, where the fastest growth rate in an exponential phase was recorded (Gr = 0.1684 log CFU mL-1 h-1, td = 1.8 h).
Abstract (croatian)
U ovom radu ocijenjena je dinamika rasta Fresco DVS 1010 kulture i humanog izolata Lactobacillus plantarum u sojinim supstratima na bazi mlijeka, vode ili bezlaktoznog mlijeka s dodatkom saharoze ili okusa (čokolada ili karamela). Statička anaerobna fermentacija provedena je tijekom 8 sati na 37 ± 0,5°C (5% CO2), nakon čega su proizvodi uskladišteni na 3 tjedna pri 6 ± 0,5°C. Iako je mlijeko tipičan medij za rast bakterija mliječne kiseline, nakon 8 h broj Fresco culture dosegao je brojku od 109 CFU mL-1. Broj potencijalno probiotičkog izolata bakterije L. plantarum dodanog nakon fermentacije ostao je iznad granice od 106 CFU mL-1 tijekom hladnog skladištenja. Nadalje, istražen je učinak temperature (8-40°C) na dinamiku rasta bakterija L. plantarum u modelu okoliša UHT bezlaktoznog mlijeka (1,5% masti). Konačna gustoća promatranog izolata dosegla je brojke 107 CFU mL-1 u stacionarnoj fazi pri svim promatranim temperaturama (osim marginalne 8°C). Tijekom rasta i razmnožavanja bakterija L. plantarum zabilježen je pad pH od početnih vrijednosti oko 0,17-1,16 jedinica kao rezultat metaboličke aktivnosti. Eksperimentalno je utvrđeno kako je optimalna temperatura za rast blizu 34°C, gdje je zabilježena najbrža stopa rasta u eksponencijalnoj fazi (Gr = 0,1684 log CFU mL-1 h-1, td = 1,8 h).
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:159:052442
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-13 13:47:40