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undergraduate thesis
Kloniranje gena ATR1, FLR1, GSH1 i YAP1 iz kvasca Saccharomyces cerevisiae soj UWOPS87-2421 u bakteriji Escherichia coli
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:890856
Arambašić, Kristian
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Arambašić, K. (2015). Kloniranje gena ATR1, FLR1, GSH1 i YAP1 iz kvasca Saccharomyces cerevisiae soj UWOPS87-2421 u bakteriji Escherichia coli (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:890856

Arambašić, Kristian. "Kloniranje gena ATR1, FLR1, GSH1 i YAP1 iz kvasca Saccharomyces cerevisiae soj UWOPS87-2421 u bakteriji Escherichia coli." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:890856

Arambašić, Kristian. "Kloniranje gena ATR1, FLR1, GSH1 i YAP1 iz kvasca Saccharomyces cerevisiae soj UWOPS87-2421 u bakteriji Escherichia coli." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:890856

Arambašić, K. (2015). 'Kloniranje gena ATR1, FLR1, GSH1 i YAP1 iz kvasca Saccharomyces cerevisiae soj UWOPS87-2421 u bakteriji Escherichia coli', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 02 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:890856

Arambašić K. Kloniranje gena ATR1, FLR1, GSH1 i YAP1 iz kvasca Saccharomyces cerevisiae soj UWOPS87-2421 u bakteriji Escherichia coli [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2024 December 02] Available at: https://urn.nsk.hr/urn:nbn:hr:159:890856

K. Arambašić, "Kloniranje gena ATR1, FLR1, GSH1 i YAP1 iz kvasca Saccharomyces cerevisiae soj UWOPS87-2421 u bakteriji Escherichia coli", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:890856

Metadata
TitleKloniranje gena ATR1, FLR1, GSH1 i YAP1 iz kvasca Saccharomyces cerevisiae soj UWOPS87-2421 u bakteriji Escherichia coli
Title (english)Cloning genes ATR1, FLR1, GSH1 i YAP1 from yeast Saccharomyces cerevisiae strain UWOPS87-2421 into bacteria Escherichia coli
AuthorKristian Arambašić
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2015-09-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Engineering
Abstract
Cilj ovog rada bio je klonirati gene ATR1, FLR1, YAP1 i GSH1 za koje je poznato da povećavaju otpornost kvasca na različite inhibitore rasta i fermentacije. Stoga su navedeni geni umnoženi lančanom reakcijom polimerazom iz soja kvasca Saccharomyces cerevisiae UWOPS87-2421 koji je otporniji na inhibitore rasta od laboratorijskih sojeva. Produkti lančane reakcije polimerazom ligirani su s odgovarajućim, prethodno lineariziranim plazmidima (pSP-G2 i pSP-G2-AC) te su ligacijske smjese korištene za transformaciju bakterije Escherichia coli. Iz transformiranih bakterija izolirani su plazmidi te je restrikcijskom analizom dokazano da su od četiri navedena gena uspješno klonirani geni ATR1, YAP1 i GSH1.
Abstract (english)
The aim of this paper was to clone genes ATR1, FLR1, YAP1 and GSH1. These genes are already known to increase the resistance of yeast Saccharomyces cerevisiae to different growth and fermentation inhibitors. Genes ATR1, FLR1, YAP1 and GSH1 were amplified by PCR from strain UWOPS87-2421 which shows higher tolerance to the inhibitors than laboratory strains. PCR products were ligated with corresponding, previously linearized plasmids (pSP-G2 and pSP-G2-AC) and ligation mixture was used to transform bacteria Escherichia coli. Restriction analysis of plasmids isolated from transformed bacteria showed that ATR1, YAP1 and GSH1 genes were successfully cloned.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:890856
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2015-12-15 13:40:06