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master's thesis
Proteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:740097
Bosanac, Petra
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Bosanac, P. (2017). Proteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:740097

Bosanac, Petra. "Proteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:740097

Bosanac, Petra. "Proteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:740097

Bosanac, P. (2017). 'Proteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:740097

Bosanac P. Proteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 June 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:740097

P. Bosanac, "Proteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:740097

Metadata
TitleProteinski izolati iz uljnih pogača lana i konoplje-priprava, karakterizacija i biološka aktivnost
Title (english)Protein isolates from flaxseed and hempseed cake - preparation, characterization and biological activity
AuthorPetra Bosanac
MentorVišnja Gaurina Srček (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2017-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Posljednjih nekoliko desetljeća prisutan je porast korištenja organskih ostataka nakon prerade proizvoda iz različitih sektora poljoprivrede i industrije. Veliki potencijal primjene imaju uljne pogače koje zaostaju nakon ekstrakcije ulja i danas se uglavnom koriste kao stočna hrana ili gnojivo. Pogače lana i konoplje sadrže različite komponente koje se smatraju vrlo važnim za ljudsko zdravlje, a značajan dio predstavljaju proteini koji mogu imati potencijal u tehnologiji životinjskih stanica. U ovom radu provedena je priprava proteinskih izolata lana i konoplje iz dva različita uzorka pri čemu je dobiven udio proteina u rasponu 43,7-60,42% za lan, odnosno 27,7-48,12% za konoplju. Ispitan je i utjecaj različitih koncentracija izolata na na proliferaciju HEK 293T stanične linije prilikom čega je utvrđeno da izolat lana nije stimulirao rast HEK 293T stanica u odnosu na standardni medij s 5% seruma,a izolat konoplje je pokazao inhibicijski učinak na rast stanica.
Abstract (english)
There has been an increased exploitation of organic residues from various sectors of agriculture and industries over the past few decades. Oil cakes, that remain after oil extraction from seeds, are mainly used as a feed source or fertilizer. Flaxseed and hempseed meal, the most interesting oil cakes today, contain various components considered to be of great importance to human health and a significant portion of proteins that can be used in animal cell technology.This thesis discusses various preparation of flax and hemp protein isolates in two different samples, whereby the protein fraction was obtained in the range of 43.7-60.42% in flax and 27.7-48.12% in the hemp protein isolate. In addition to flaxseed and hempseed meal, human HEK 293T cell line derived from the transformation of human embryonic kidney cells with DNA adenovirus 5 was also used. The influence of different isolate concentrations on the proliferation of HEK 293T cell line was determined, whereby the growth of HEK 293T cells was not stimulated by flax protein isolate in compare to the standard medium with 5% serum, and also, a hemp protein isolate showed an inhibitory effect on cell growth.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:740097
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-16 10:41:11