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master's thesis
Utjecaj metode izolacije DNA na određen sastav intestinalne mikrobiote
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:806794
Ćutić, Maja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Section for Bioinformatics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Ćutić, M. (2017). Utjecaj metode izolacije DNA na određen sastav intestinalne mikrobiote (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:806794

Ćutić, Maja. "Utjecaj metode izolacije DNA na određen sastav intestinalne mikrobiote." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:806794

Ćutić, Maja. "Utjecaj metode izolacije DNA na određen sastav intestinalne mikrobiote." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:806794

Ćutić, M. (2017). 'Utjecaj metode izolacije DNA na određen sastav intestinalne mikrobiote', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 08 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:806794

Ćutić M. Utjecaj metode izolacije DNA na određen sastav intestinalne mikrobiote [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 May 08] Available at: https://urn.nsk.hr/urn:nbn:hr:159:806794

M. Ćutić, "Utjecaj metode izolacije DNA na određen sastav intestinalne mikrobiote", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:806794

Metadata
TitleUtjecaj metode izolacije DNA na određen sastav intestinalne mikrobiote
Title (english)The effect of DNA extraction method on the gut microbiota composition
AuthorMaja Ćutić
MentorJurica Žučko (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Section for Bioinformatics)
Zagreb
Defense date and country2017-05-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Crijevna mikrobiota predstavlja relativno novo, zanimljivo područje istraživanja povezano s mnogim stanjima ljudskog organizma, od održavanja homeostaze preko povremenih gastrointestinalnih poremećaja, do povezanosti s bolestima. Povezanost s pretilošću omogućava bolji uvid u prevenciju, razvoj i liječenje ove bolesti čija incidencija kontinuirano raste. Kako bi se bolje razumjela ta povezanost, koriste se suvremene metode analize koje uključuju sekvencioniranje bakterijskog 16S rRNA gena iz uzorka fecesa. Cilj ovog rada bio je usporediti tri metode za izolaciju DNA iz uzorka fecesa pretile osobe kako bi se vidjele razlike u dobivenim podacima i utjecaj ovog pretkoraka na daljnje analize. Također, promatrao se utjecaj dodavanja DNA ShieldTM stabilizatora. Obradom podataka pomoću QIIME bioinformatičkog alata, dobiveni su podaci o DNA prinosu i bioraznolikosti uzoraka u ovisnosti o metodi izolacije. Za daljna istraživanja poželjno je koristiti jednu metodu kako bi se smanjila pogreška odnosno dobili sastavi mikrobiote koji su međusobno usporedivi.
Abstract (english)
Human gut microbiota is rapidly becoming one of the most interesting fields of studies regarding health and disease development. Emerging evidences show relationship between gut microbiota and obesity, one of the fastest delevoping diseases worldwide. To better understand this connection, methods such as 16S rRNA sequencing are applied. The aim of this study was to compare three available methods of DNA extraction that were used to extract DNA from faeces of a obese person. Data collected from sequencing were processed using QIIME bioinformatical tool in order to observe differences in bacterial diversity and DNA yield depending on DNA extraction method. The influence of applying DNA ShieldTM reagens was also observed. Io order to decrease biases in studies regarding analysis of the gut microbiota, it is recommended to use one specific method of DNA extraction.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:806794
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-11-23 10:35:30