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master's thesis
Proizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:641134
Galić, Maja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Galić, M. (2017). Proizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:641134

Galić, Maja. "Proizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:641134

Galić, Maja. "Proizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:641134

Galić, M. (2017). 'Proizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:641134

Galić M. Proizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 August 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:641134

M. Galić, "Proizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:641134

Metadata
TitleProizvodnja bioetanola na blago kiselinskom hidrolizatu pšenične slame dobivenom pomoću fosforne kiseline u različitim mjerilima
Title (english)Bioethanol production on the mild acid hydrolysates of wheat straw obtained by phosphoric acid at various scales
AuthorMaja Galić
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2017-09-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Bioetanol je važan obnovljiv izvor energije koji ima veliki potencijal za zamjenu fosilnih transportnih goriva. Velike količine jeftinih industrijskih i poljoprivrednih ostataka idealne su sirovine za proizvodnju bietanola pri čemu se njihovim zbrinjavanjem sprječava zagađenje okoliša. Pšenična slama je dobar primjer takve sirovine koji se može primjeniti u procesu proizvodnje bioetanola. Međutim, pšenična slama mora proći proces predobrade da bi se mogla primjeniti u procesu proizvodnje bioetanola. U ovom istraživanju proučavan je proces blago kisele hidrolize pomoću fosforne kiseline u visokotlačnom reaktoru pri različitim kombinacijama parametara procesa predobrade. Proučavane su različite kombinacije svih parametara procesa predobrade: temperatura (160 – 200 °C), vrijeme zadržavanja (1 - 10 minuta) i koncentracije fosforne kiseline (0,5 – 2,0 %). Najveća koncentracija fermentabilnih šećera zabilježena je u tekućem dijelu hidrolizata pšenične slame kod predobrade na 180 °C, vremenu zadržavanja od 10 minuta i koncentraciji H3PO4 od 2,0 %. U čvrstom dijelu hidrolizata pšenične slame kod prethodno navedenih uvjeta detektirani su ovi sastojci: glukan (42,43 %), ksilan (19,64 %), arabinan (9,01 %). Dobiveni hidrolizati pšenične slame korišteni su za anaerobni uzgoj divljeg i genetički modificiranog soja (Y9) kvasca S. cerevisiae u Erlenmeyer tikvicama i horizontalno rotirajućem cijevnom bioreaktoru. Najuspješniji anaerobni uzgoj genetički modificiranog soja kvasca S. cerevisiae Y9 proveden je na hidrolizatu pšenične slame dobivenom pri temperaturi od 180 °C, vremenu zadržavanja od 10 minuta i koncentraciji fosforne kiseline od 2,0 % u HRCB-u pri čemu su dobiveni ovi pokazatelji uspješnosti bioprocesa: Pr: 0,0057 gL-1h-1, YP/S: 0,058 gg-1, E: 11,34 %.
Abstract (english)
Bioethanol is an important renewable energy source that has great potential to replace fossil transportation fuels. Large quantities of cheap industrial and agricultural residues are the ideal raw materials for the production of bioethanol, whereby their disposal prevents environmental pollution. Wheat straw is a good example of such raw materials that can be applied in the production process of bioethanol. However, wheat straw has to go through the process of pretreatment that could be applied in the production process of bioethanol. In this study, the process of mild acid hydrolysis by phosphoric acid in a high - pressure reactor at various combinations of processing parameters was studied. Various combinations of all processing parameters: temperature (160 - 200 °C), holding time (1 - 10 minutes) and phosphoric acid concentration (0.5 - 2.0%) were studied. The highest concentration of fermentable sugar was observed in the liquid hydrolyzate of wheat straw pretreated at 180 °C, residence time of 10 minutes and a concentration of 2,0 % H3PO4. In the solid hydrolyzate of wheat straw, at previous conditions, these components are detected: glucan (42,43 %), xylan (19,64 %), arabinan (9,01 %). The obtained hydrolyzate of wheat straw was used for anaerobic cultivation of wild and genetically modified yeast S. cerevisiae, Y9 in Erlenmeyer flasks and horizontally rotating tubular bioreactor. The most successful anaerobic cultivation of genetically modified yeast S. cerevisiae, Y9 was carried out on the hydrolyzate of wheat straw obtained at 180 °C, 10 minute retention time and 2.0% phosphoric acid concentration in HRCB, where these bioprocess performance indicators were obtained: Pr: 0.0057 gL-1h-1, YP / S: 0.058 gg-1, E: 11.34%.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:641134
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2017-11-23 14:24:50