prikaz prve stranice dokumenta Primjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima
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doctoral thesis
Primjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2006. urn:nbn:hr:159:911688
Kovaček, Ivančica
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kovaček, I. (2006). Primjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:911688

Kovaček, Ivančica. "Primjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2006. https://urn.nsk.hr/urn:nbn:hr:159:911688

Kovaček, Ivančica. "Primjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2006. https://urn.nsk.hr/urn:nbn:hr:159:911688

Kovaček, I. (2006). 'Primjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 07 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:911688

Kovaček I. Primjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2006 [cited 2025 January 07] Available at: https://urn.nsk.hr/urn:nbn:hr:159:911688

I. Kovaček, "Primjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2006. Available at: https://urn.nsk.hr/urn:nbn:hr:159:911688

Metadata
TitlePrimjena imunomagnetske separacije u istraživanju rasta odabranih patogenih mikroorganizama u mlijeku i mliječnim proizvodima
Title (english)Use of immunomagnetic separation in researching growth of certain pathogenic microorganisms in milk and dairy products
AuthorIvančica Kovaček
MBZ: 175515
MentorFrane Delaš (mentor)
MBZ: 9173
Committee memberSenadin Duraković (predsjednik povjerenstva)
MBZ: 10973
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2006-04-12, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Food Technology
Universal decimal classification
(UDC)		663 - Industrial microbiology. Industrial mycology. Zymurgy, fermentation industry. Beverage industry. Stimulant industry
Abstract
Tradicionalne metode uzgoja i izolacije patogenih bakterija zahtijevaju puno vremena i opterećuju rutinske laboratorije koji zbog toga pokušavaju uvesti nove metode s pomoću kojih se u kraćem vremenu može otkriti mala količina odabranih patogenih bakterija. Svrha je disertacije usporedba nove metode imunomagnetske separacije s tradicionalnim metodama uzgoja i izolacije odabranih patogenih bakterija Salmonella spp., Listeria monocytogenes i Escherichia coli O157:H7 i kako se primjenom te metode mogu unaprijediti postojeće metode. Imunomagnetska separacija (IMS) pokazala se osjetljivom i specifičnom u izolaciji Escherichia coli O157:H7. U izolaciji Salmonella spp. jednako je osjetljiva kao i tradicionalna metoda, a nije dovoljno osjetljiva za izolaciju Listeria monocytogenes. Za tu patogenu bakteriju boljom se pokazala tradicionalna metoda uzgoja. Ipak, metoda imunomagnetske separacije skraćuje vrijeme potrebno za izolaciju i može se upotrijebiti kao iznimno korisna uz tradicionalne metode.
Abstract (english)
Conventional techniques of cultivation and isolation involve pre-enrichment and enrichment broths for recovery of stressed bacteria, colony isolation on selective hard food bases, application of biochemical tests and serotyping using antibodies against specific bacterial antigens. These procedures are time consuming and cumbersome because it takes several days to establish the identity of a particular bacteria. Therefore, new approaches are needed for fast and efficient detection of low numbers of bacteria. The objective of this dissertation was to compare the new method of immunomagnetic separation with traditional isolation methods using cultivation on several chosen pathogenic bacteria; Salmonella spp., Listeria monocytogenes and Escherichia coli O157:H7. Immunomagnetic separation (IMS) has proven to be particularly sensitive to and specific for the isolation of Escherichia coli O157:H7, as sensitive as the classic methods in the isolation of Salmonella spp., but lacking the necessary sensitivity in the isolation of Listeria monocytogenes. For this pathogen, conventional cultural techniques were more sensitive and therefore more efficient in isolation than immunomagnetic separation. However, immunomagnetic separation was very specific in the isolation of determined species, and this, coupled with its processing speed, means it could indeed be used as a very useful method to support the conventional methods.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:911688
Promotion2006-05-31
Study programmeTitle: Postgraduate PhD Study in Food Technology
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti, područje biotehničkih znanosti, polje prehrambena tehnologija (doktor/doktorica znanosti, područje biotehničkih znanosti, polje prehrambena tehnologija)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-05-11 12:03:51