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doctoral thesis
Antifungalni uĉinak eteriĉnih ulja na plijesni iz roda Aspergillus u in vitro i in situ uvjetima
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. urn:nbn:hr:159:648569
Sokolić-Mihalak, Darja
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Sokolić Mihalak, D. (2013). Antifungalni uĉinak eteriĉnih ulja na plijesni iz roda Aspergillus u in vitro i in situ uvjetima (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:648569

Sokolić Mihalak, Darja. "Antifungalni uĉinak eteriĉnih ulja na plijesni iz roda Aspergillus u in vitro i in situ uvjetima." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:648569

Sokolić Mihalak, Darja. "Antifungalni uĉinak eteriĉnih ulja na plijesni iz roda Aspergillus u in vitro i in situ uvjetima." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2013. https://urn.nsk.hr/urn:nbn:hr:159:648569

Sokolić Mihalak, D. (2013). 'Antifungalni uĉinak eteriĉnih ulja na plijesni iz roda Aspergillus u in vitro i in situ uvjetima', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:648569

Sokolić Mihalak D. Antifungalni uĉinak eteriĉnih ulja na plijesni iz roda Aspergillus u in vitro i in situ uvjetima [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2013 [cited 2024 November 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:648569

D. Sokolić Mihalak, "Antifungalni uĉinak eteriĉnih ulja na plijesni iz roda Aspergillus u in vitro i in situ uvjetima", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2013. Available at: https://urn.nsk.hr/urn:nbn:hr:159:648569

Metadata
TitleAntifungalni uĉinak eteriĉnih ulja na plijesni iz roda Aspergillus u in vitro i in situ uvjetima
Title (english)Antifungal effect of essential oils in vitro and in situ on Aspergillus species
AuthorDarja Sokolić-Mihalak
MentorHrvoje Pavlović (mentor)
Committee memberFrane Delaš (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2013-01-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Universal decimal classification
(UDC)		60 - Biotechnology. Genetic engineering. Products of cloning
Abstract
Cilj ovog rada bio je ispitati antifungalna svojstva eteričnih ulja majčine dušice, čajevca i cedra, te njihov utjecaj na sintezu mikotoksina, u cilju potencijalne mogućnosti uporabe kao ekološki prihvatljivih konzervansa u zaštiti hrane od kvarenja plijesnima Aspergillus carbonarius, Aspergillus flavus i Aspergillus parasiticus. OdreĎivanjem minimalne inhibitorne koncentracije (MIK) i minimalne fungicidne koncentracije (MFK), te odreĎivanjem učinka eteričnih ulja majčine dušice, cedra i čajevca na inhibiciju linearnog rasta kolonija plijesni pri različitim aktivitetima vode, ustanovljeno je kako pri oba aktiviteta vode i koncentraciji od 500 ppm eterično ulje majčine dušice u potpunosti inhibira rast plijesni A. carbonarius. Ispitivanje učinka eteričnih ulja na inhibiciju germinacije spora pokazalo je da najveći utjecaj na smanjenje germinacije spora plijesni A. flavus ima eterično ulje čajevca, a eterično ulje majčine dušice na germinaciju spora plijesni A. parasiticus. Na smanjenje sinteze mikotoksina za sve ispitivane plijesni najznačajniji utjecaj pokazalo je eterično ulje majčine dušice. Ispitivanjem u realnim uvjetima na kolutićima mrkve najveći inhibitorni učinak porasta kolonija plijesni i to na plijesan A. flavus pokazalo je eterično ulje majčine dušice. Svi dobiveni rezultati upućuju na to da od svih ispitivanih eteričnih ulja, eterično ulje majčine dušice ima najveći potencijal za korištenje u sprečavanju kvarenja hrane pri koncentraciji od 250 – 500 ppm i smanjenom aktivitetu vode.
Abstract (english)
The aim of this study was to investigate the antifungal properties of essential oils of wild thyme, tea tree and cedar, and their impact on mycotoxins in order to possible use as an environmentally friendly preservatives in protecting food from spoilage molds of Aspergillus carbonarius, Aspergillus flavus and Aspergillus parasiticus. Determination of minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC), and determining the effect of essential oils of wild thyme, tea tree and cedar on the inhibition of linear growth of mold colonies at different water activity, it was found that both the water activity and the concentration of 500 ppm of essential oil of wild thyme completely inhibited A. carbonarius mold growth. Testing the effect of essential oils on spore germination inhibition showed that the greatest impact on reducing germination spores of A. flavus has tea tree essential oil, essential oil of wild thyme on the germination of spores A. parasiticus. The decrease in the synthesis of mycotoxins for all mold testing showed the most significant effect is an essential oil of wild thyme. Testing under real conditions on carrot largest colony growth inhibitory effects of mold of the genus A. flavus showed the essential oil of wild thyme. All results indicate that all of the tested essential oils, essential oil of wild thyme has the greatest potential for use in preventing food spoilage at concentrations 250-500 ppm and reduced water activity.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:648569
Promotion2013-09-22
Study programmeTitle: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-05-16 11:35:28