prikaz prve stranice dokumenta Utjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora
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doctoral thesis
Utjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. urn:nbn:hr:159:235346
Tršan, Tihana
University of Zagreb
Faculty of Food Technology and Biotechnology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Tršan, T. (2015). Utjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora (Doctoral thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:235346

Tršan, Tihana. "Utjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:235346

Tršan, Tihana. "Utjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora." Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2015. https://urn.nsk.hr/urn:nbn:hr:159:235346

Tršan, T. (2015). 'Utjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora', Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 18 February 2025, https://urn.nsk.hr/urn:nbn:hr:159:235346

Tršan T. Utjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora [Doctoral thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2015 [cited 2025 February 18] Available at: https://urn.nsk.hr/urn:nbn:hr:159:235346

T. Tršan, "Utjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora", Doctoral thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2015. Available at: https://urn.nsk.hr/urn:nbn:hr:159:235346

Metadata
TitleUtjecaj ekspresije liganda za receptor NKG2D na potencijal citomegalovirusa kao vakcinskoga vektora
Title (english)The role of NKG2D ligand expression by cytomegalovirus on its vaccine-vector capacity
AuthorTihana Tršan
MentorStipan Jonjić (mentor)
Committee memberJagoda Šušković (predsjednik povjerenstva)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
Zagreb
Defense date and country2015-02-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Universal decimal classification
(UDC)		578/579 - Virology. Microbiology
Abstract
Iako vakcinacija predstavlja najuspješniji pristup zaštite protiv patogena, učinkovite vakcine protiv značajnog broja patogena još uvijek nisu dostupne. Stoga su neophodni novi pristupi u dizajnu i proizvodnji vakcina. Živi atenuirani citomegalovirus (CMV) je atraktivan vakcinski vektor s obzirom na snažan odgovor posredovan CD8 T-limfocitima koji potiče u domaćinu. CMV je razvio brojne strategije izbjegavanja imunosnog odgovora domaćina, a posebice kontrole posredovane NKG2D receptorom. NKG2D djeluje kao snažan aktivacijski receptor kada je eksprimiran na NK stanicama te kao ko-stimulacijski receptor na CD8 T-limfocitima. U ovom radu pokazano je da mišji CMV (MCMV) koji eksprimira NKG2D ligand RAE-1γ služi kao izuzetno učinkovit vakcinski vektor. Koekspresija RAE-1γ i antigenskog epitopa od strane MCMV-a pojačala je odgovor posredovan CD8 T-limfocitima specifičnim za vektorski epitop i rezultirala dugotrajnom zaštitom u infekciji patogenom posredovanom upravo epitop-specifičnim CD8 T-limfocitima. MCMV vektor koji eksprimira RAE-1γ ligand bio je snažno atenuiran i jednako protektivan čak i u jedinki koje ne eksprimiraju NKG2D receptor na svojim stanicama, što ukazuje na novu, do sada nepoznatu i NKG2D neovisnu, funkciju RAE-1γ proteina.
Abstract (english)
Although vaccination proved to be successful method against various pathogens, efficient vaccine for a significant number of diseases is still not available. Therefore, new vaccine strategies are required. Live attenuated cytomegalovirus (CMV) is attractive vaccine-vector candidate due to the potent CD8 T cell response it triggers. CMV developed numerous strategies to avoid immune control of the host, especially the one mediated by the NKG2D receptor - strong activating receptor when expressed on NK cells or co-stimulatory molecule on CD8 T cells. It is demonstrated here that mouse CMV (MCMV) expressing NKG2D ligand RAE-1γ possesses the exceptional capacity to serve as a highly efficient vaccine-vector. Co-expression of RAE-1γ and foreign CD8 T cell epitope in the context of MCMV augmented epitope-specific CD8 T cell response and resulted in long-term protection against respective pathogen by means of CD8 T cells specific to the vectored epitope. RAE-1γ expressing MCMV vector was highly attenuated and equally protective even in NKG2D deficient mice, implying the existence of additional, so far unknown and NKG2D-independent, immune function of RAE1γ.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:235346
Promotion2015-07-12
Study programmeTitle: Postgraduate PhD Study in Biotechnology and Bioprocess Engineering
Study programme type: university
Study level: postgraduate
Academic / professional title: doktor/doktorica znanosti (doktor/doktorica znanosti)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2018-05-18 08:32:22