The most toxic representative of ochratoxin group is the ochratoxin A (OTA). As a secondary metabolite of molds of the genera Aspergillus and Penicillium, it is a powerful toxin with hepatotoxic, nephrotoxic, carcinogenic and immunosuppressive activity. Estimated dietary exposure to ochratoxin A in the EU is 0.13 to 4.6 ng / kg body weight per day. Wine is secondary source of human exposure to ochratoxin A in Europe. For proper laboratory analysis of mycotoxins, it may be necessary to meet certain parameters relating to the validation of methods intended for mycotoxin analysis. Besides the official analytical method validation for mycotoxins based on the chromatograpy principles, today orientation tests have become a standard tool for fast analysis of ochratoxin A, particularly for the purposes of monitoring. Such assay is an ELISA (Enzyme - linked immunosorbent assay) test. Despite the strong influence of the matrix and false-positive results, the advantages of ELISA are speed, ease of operation and satisfactory sensitivity of the method. In this study, the presence of ochratoxin A in samples of white, red and dessert wines was examined. Samples of wine were taken from the market of Bosnia and Herzegovina as well as from the domestic wine producers in Herzegovina during the 2012 and 2013 year. This study also shows the quality of test samples for the presence of ochratoxin A in wines through parameters of method validation. Efficacy of ELISA technique as a reliable method for quantifying a large number of samples was proven. The resulting amounts of ochratoxin A were compared with the EU maximum allowed concentrations for wine, and it was found that the analyzed samples were safe and, as such, may be placed on the market.