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undergraduate thesis
Izolacija i pročišćavanje egzopolisaharida soja Lactobacillus fermentum D12 nakon uzgoja u prisutnosti fruktoze i galaktoze kao izvora ugljika
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:695544
Pušek, Anita
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Pušek, A. (2018). Izolacija i pročišćavanje egzopolisaharida soja Lactobacillus fermentum D12 nakon uzgoja u prisutnosti fruktoze i galaktoze kao izvora ugljika (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:695544

Pušek, Anita. "Izolacija i pročišćavanje egzopolisaharida soja Lactobacillus fermentum D12 nakon uzgoja u prisutnosti fruktoze i galaktoze kao izvora ugljika." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:695544

Pušek, Anita. "Izolacija i pročišćavanje egzopolisaharida soja Lactobacillus fermentum D12 nakon uzgoja u prisutnosti fruktoze i galaktoze kao izvora ugljika." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:695544

Pušek, A. (2018). 'Izolacija i pročišćavanje egzopolisaharida soja Lactobacillus fermentum D12 nakon uzgoja u prisutnosti fruktoze i galaktoze kao izvora ugljika', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 24 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:695544

Pušek A. Izolacija i pročišćavanje egzopolisaharida soja Lactobacillus fermentum D12 nakon uzgoja u prisutnosti fruktoze i galaktoze kao izvora ugljika [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 July 24] Available at: https://urn.nsk.hr/urn:nbn:hr:159:695544

A. Pušek, "Izolacija i pročišćavanje egzopolisaharida soja Lactobacillus fermentum D12 nakon uzgoja u prisutnosti fruktoze i galaktoze kao izvora ugljika", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:695544

Metadata
TitleIzolacija i pročišćavanje egzopolisaharida soja Lactobacillus fermentum D12 nakon uzgoja u prisutnosti fruktoze i galaktoze kao izvora ugljika
Title (english)Isolation and purification of the exopolisaccharides of Lactobacillus fermentum D12 in the presence of fructose and galactose as a carbon sources
AuthorAnita Pušek
MentorAndreja Leboš Pavunc (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2018-06-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U proizvodnji egzopolisaharida (EPOL) u posljednje vrijeme se posebno istražuju bakterije mliječne kiseline (BMK) s ciljem poboljšanja svojstava proizvoda i unaprjeđenja kvalitete ljudskog zdravlja. Također, neki sojevi bakterija mliječne kiseline sintezom egzopolisaharida pridonose poboljšanju teksture i viskoznosti fermentiranih proizvoda. Stoga je cilj ovog završnog rada bio ispitati sposobnost različitih sojeva BMK da sintetiziraju egzopolisaharide u MRS hranjivoj podlozi suplementiranoj s različitim izvorima ugljika (2% w/v fruktoza, galaktoza). Soj Lactobacillus fermentum D12 se pokazao kao najbolji producent egzopolisaharida. Nakon provedenog uzgoja L. fermentum D12, slijedila je izolacija egzopolisaharida s površine stanica L. fermentum D12 te iz supernatanta ove bakterijske kulture nakon čega je izračunata masa egzopolisaharida. Čistoća egzopolisaharida na prisutnost proteina se provjerila UV-spektrofotometrijom pri valnoj duljini od 190 do 350 nm. Koncentracija prisutnih proteina kod uzoraka egzopolisaharida izoliranih s površine stanica bila je puno veća, u odnosu na one izolirane iz supernatanta kulture.
Abstract (english)
In the recent years, lactic acid bacteria (LAB) have been specifically investigated in the frame of the exopolysaccharide production, because of its impact in improving product properties and improving the quality of human health. In addition, several lactic acid bacterial species, that synthesis exopolisaccharides, contribute to improving texture and viscosity of fermented products. Therefore, the aim of this final study was to examine the ability of several LAB to synthesis exopolisaccharides in MRS nutrient medium supplemented with different carbon sources (2% w/v fructose, galactose). Lactobacillus fermentum D12 has been shown, as the best exopolisaccharides producer among tested strains. After producing cultivation of L. fermentum D12, isolation and mass calculation of the exopolisaccharides from the cell surface and from the culture supernatant, was performed. The purity of the exopolisaccarides on the presence of the proteins was verified by UV spectrophotometry at wavelenngthsfrom 190 to 350 nm. The concentration of proteins of exopolisaccharides samples isolated from the surface of the cell was much higher than those isolated from the culture supernatant.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:695544
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-06-24 10:11:00