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undergraduate thesis
Razvoj metode za fluorimetrijsko određivanje klorofila a
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:547434
Smoljo, Josipa
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Physical Chemistry and Corrosion

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Smoljo, J. (2018). Razvoj metode za fluorimetrijsko određivanje klorofila a (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:547434

Smoljo, Josipa. "Razvoj metode za fluorimetrijsko određivanje klorofila a." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:547434

Smoljo, Josipa. "Razvoj metode za fluorimetrijsko određivanje klorofila a." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:547434

Smoljo, J. (2018). 'Razvoj metode za fluorimetrijsko određivanje klorofila a', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:547434

Smoljo J. Razvoj metode za fluorimetrijsko određivanje klorofila a [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:547434

J. Smoljo, "Razvoj metode za fluorimetrijsko određivanje klorofila a", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:547434

Metadata
TitleRazvoj metode za fluorimetrijsko određivanje klorofila a
Title (english)The development of a fluorimetric method for the determination of chlorophyll a
AuthorJosipa Smoljo
MentorMojca Čakić Semenčić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Physical Chemistry and Corrosion)
Zagreb
Defense date and country2018-07-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Klorofil a je zeleni pigment prisutan u biljkama, cijanobakterijama i algama te je ključan u procesu fotosinteze. U prehrambenoj industriji njegovi se derivati koriste kao prirodna bojila biljnoga podrijetla, a u posljednje vrijeme raste broj literaturnih primjera u kojima se klorofilu a pripisuje antikancerogena, antimutagena i antioksidativna aktivnost. Koncentracija klorofila a u biljnim ekstraktima u laboratoriju se rutinski određuje mjerenjem apsorbancije pri apsorpcijskom maksimumu klorofila a (660 nm) te primjenom empirijskih jednadžbi. Imajući u vidu veću osjetljivost i selektivnost fluorimetrije kao analitičke metode u odnosu na UV/ViS spektrofotometriju te dvojbenu pouzdanost empirijskih jednadžbi, cilj ovoga rada bio je razvoj metode za fluorimetrijsko određivanje koncentracije klorofila a u biljnim ekstraktima. Standard klorofila a izoliran je iz cijanobakterije Arthrospira platensis primjenom tankoslojne preparativne kromatografije.
Abstract (english)
Chlorophyll a is a green pigment present in plants, cyanobacteria and algae and is crucial in the photosynthesis process. Besides the usage of its derivatives as natural dyes of plant origin in food industry, there is a growing number of literary examples in which anticancer, antimutagenic and antioxidant activity is attributed to chlorophyll a. Concentration of chlorophyll an in plant extracts is routinely determined in the laboratory by measuring the absorbance at the absorption maximum of chlorophyll a (660 nm) and using empirical equations. Having in mind greater sensitivity and selectivity of fluorimetry as analytical method compared to UV/ViS spectrophotometry and reliability of empirical equations, the aim of this study was to develop a method for fluorimetric determination of chlorophyll a concentration in plant extracts. Chlorophyll a used as standard was isolated from cyanobacteria Arthrospira platensis by preparative thin layer chromatography.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:547434
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-07-18 08:38:51