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master's thesis
Biološka aktivnost spojeva iz repičinog ulja
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. urn:nbn:hr:159:165524
Brkan, Veronika
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Brkan, V. (2016). Biološka aktivnost spojeva iz repičinog ulja (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:165524

Brkan, Veronika. "Biološka aktivnost spojeva iz repičinog ulja." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:165524

Brkan, Veronika. "Biološka aktivnost spojeva iz repičinog ulja." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2016. https://urn.nsk.hr/urn:nbn:hr:159:165524

Brkan, V. (2016). 'Biološka aktivnost spojeva iz repičinog ulja', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:165524

Brkan V. Biološka aktivnost spojeva iz repičinog ulja [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2016 [cited 2024 November 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:165524

V. Brkan, "Biološka aktivnost spojeva iz repičinog ulja", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2016. Available at: https://urn.nsk.hr/urn:nbn:hr:159:165524

Metadata
TitleBiološka aktivnost spojeva iz repičinog ulja
Title (english)The biological activity of compounds from rapeseed oil
AuthorVeronika Brkan
MentorKristina Radošević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2016-06-02, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Uljana repica i njeno ulje izvor su mnogih biološki aktivnih spojeva, osobito fenolnih spojeva za koje je potvrđeno da imaju antioksidacijsko, antimutageno i antitumorsko djelovanje. Iz sirovog ulja izoliran je 4-vinil-2,6-dimetoksifenol ili kanolol, a iz jestivog njegov dimer, fenilindan. U ovom radu ispitana je biološka aktivnost navedenih spojeva određivanjem antioksidacijskog kapaciteta ORAC metodom te in vitro testom citotoksičnosti na humanim tumorskim staničnim linijama (MCF-7, HeLa) te na normalnoj staničnoj liniji sisavaca (HEK293T). Ispitano je mogu li kanolol i fenilindan imati zaštitni učinak na stanice u kojima je izavan oksidativni stres. Rezultati istraživanja pokazali su citotoksičan učinak kanolola i fenilindana na sve tri stanične linije, s tim da fenilindan ima jači citotoksični učinak. Također, fenilindan ima veći antioksidacijski potencijal nego kanolol. Nije zabilježen zaštitni učinak kanolola i fenilindana na MCF-7, HeLa i HEK293T stanice u kojima je potaknut oksidativni stres.
Abstract (english)
Rapeseed and its oil are the source of many biologically active compounds, especially phenolic compounds that have been confirmed to have antioxidant, antimutagenic and anticancer activity. From crude oil is isolated 4-vinyl-2,6-dimethoksiphenol or canolol and from edible oil its dimer, phenylindan. In this study, we tested the biological activity of these compounds by determination of the antioxidant capacity by ORAC method and in vitro cytotoxicity in human tumor cell lines (MCF-7, HeLa) and in normal mammalian cell line (HEK). We examined whether canolol and phenylindane have a protective effect on the cells with induced oxidative stress. The results showed a cytotoxic effect of canololand phenylindane on all three cell lines, however phenylindan has a stronger cytotoxic effect. Also, phenylindane has a greater antioxidant potential than canolol. There had been no record of protective effect of canolol and phenylindane on the cells with induced oxidative stress.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:165524
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2017-04-26 07:41:47