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undergraduate thesis
Mikrobiota majčinog mlijeka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:672700
Cavrić, Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Cavrić, M. (2018). Mikrobiota majčinog mlijeka (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:672700

Cavrić, Marija. "Mikrobiota majčinog mlijeka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:672700

Cavrić, Marija. "Mikrobiota majčinog mlijeka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:672700

Cavrić, M. (2018). 'Mikrobiota majčinog mlijeka', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:672700

Cavrić M. Mikrobiota majčinog mlijeka [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:672700

M. Cavrić, "Mikrobiota majčinog mlijeka", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:672700

Metadata
TitleMikrobiota majčinog mlijeka
Title (english)Human milk microbiota
AuthorMarija Cavrić
MentorJagoda Šušković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2018-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Majčino mlijeko sadrži nekoliko tisuća bakterijskih vrsta u koncentracijama od približno 1000 CFU mL-1. Procjenjuje se da dojenče koje konzumira oko 800 mL majčinog mlijeka dnevno unese oko 105-107 komensalnih bakterija, zbog čega postoji sličnost u sastavu mikrobiote majčinog mlijeka s mikrobiotom crijeva djeteta. U ovom radu provedeno je identificiranje mikrobiote majčinog mlijeka sa po 3 uzorka mlijeka svake majke uzetih u različito vrijeme nakon poroda i fecesa dojenčadi sa 3 uzorka uzetih isti dan kao i uzorci mlijeka. Nakon izolacije DNA iz uzoraka majčinog mlijeka i fecesa dojenčadi, uzorci DNA su sekvencionirani te analizirani pomoću QIIME programa za analizu mikrobioma. Nakon utvrđivanja taksonomskih jedinica u uzorcima, određena je i alfa raznolikost. Dobivenim rezultatima utvrđena je korelacija sastava mikrobioma majčinog mlijeka s neonatalnim mikrobiomom dojenčeta. Najprisutnije bakterije u uzorcima fecesa dojenčadi su iz koljena Actinobacteria, Firmicutes i Proteobacteria, dok u uzorcima majčinog mlijeka prevladavaju bakterije iz roda Firmicutes.
Abstract (english)
Breast milk contains several hundred bacterial species at concentrations of aproximately 1000 CFU mL-1. It is estimated that infants who consume about 800 mL of mother's milk daily, ingest 105-107 commensal bacteria, which explains a similarity in the composition of human milk microbiota with infants intestinal microbiota. In this Bachelor thesis, the identification of human milk microbiota with 3 samples of milk from each mother sampled at different times after delivery and 3 samples of infants feces taken the same day as milk samples, was performed. After DNA isolation, DNA samples were sequenced and analyzed using QIIME program for microbiome analyses. After determing the taxonomy units in the samples, alpha diversity was also determined. With given results, correlation between breast milk microbiome and neonatal intestinal microbiome was concluded. Infants feces samples are dominated by the phyla Actinobacteria, Firmicutes and Proteobacteria, while breast milk samples are dominated by Firmicutes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:672700
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-07-19 08:37:33