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undergraduate thesis
Rezistencija transformiranih sojeva kvasca s pojačanom ekspresijom odabranih gena na inhibitore rasta
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:849682
Moguš, Leo
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Moguš, L. (2018). Rezistencija transformiranih sojeva kvasca s pojačanom ekspresijom odabranih gena na inhibitore rasta (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:849682

Moguš, Leo. "Rezistencija transformiranih sojeva kvasca s pojačanom ekspresijom odabranih gena na inhibitore rasta." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:849682

Moguš, Leo. "Rezistencija transformiranih sojeva kvasca s pojačanom ekspresijom odabranih gena na inhibitore rasta." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:849682

Moguš, L. (2018). 'Rezistencija transformiranih sojeva kvasca s pojačanom ekspresijom odabranih gena na inhibitore rasta', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 15 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:849682

Moguš L. Rezistencija transformiranih sojeva kvasca s pojačanom ekspresijom odabranih gena na inhibitore rasta [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 July 15] Available at: https://urn.nsk.hr/urn:nbn:hr:159:849682

L. Moguš, "Rezistencija transformiranih sojeva kvasca s pojačanom ekspresijom odabranih gena na inhibitore rasta", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:849682

Metadata
TitleRezistencija transformiranih sojeva kvasca s pojačanom ekspresijom odabranih gena na inhibitore rasta
Title (english)Resistance of transformed yeast breeds with overexpression of selected genes on growth inhibitors
AuthorLeo Moguš
MentorIvan-Krešimir Svetec (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2018-07-16, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Jedan od pravaca razvoja u svrhu zadovoljavanja stalno rastućih potreba za energijom je proizvodnja biogoriva odnosno bioetanola iz otpadnih sirovina poljoprivrede, šumarstva i drvoprerađivačke industrije. Ove lignocelulozne sirovine su izvor jednostavnih šećera koji se fermentiraju u etanol. U ovim bioprocesima, zbog svojih mnogobrojnih prednosti, radni mikroorganizam je kvasac Saccharomyces cerevisiae. Međutim, spojevi koje kvasac može fermentirati, iz lignoceluloznih sirovina se dobivaju predobradom sirovine, tijekom koje nastaju i inhibitori rasta i fermentacije, kao što su octena i levulinska kiselina te 2-furaldehid. Budući da ovi spojevi uvelike otežavaju proizvodnju bioetanola, mnoga istraživanja su usmjerena prema konstrukciji novih sojeva kvasca, otpornijih na inhibitore rasta i fermentacije. Zbog toga je u ovom radu istražen utjecaj ekspresije osam gena na otpornost soja kvasca, izoliranog Sladorane d.o.o. Županja, prema pojedinim inhibitorima rasta. Dobiveni rezultati ukazuju na zaključak da pojačana ekspresija gena YAP1 i CTA1 doprinosi otpornosti kvasca na levulinsku kiselinu i 2-furaldehid te da na otpornost kvasca prema 2-furaldehidu pozitivno utječe i pojačana ekspresija gena ATR1 i GSH1.
Abstract (english)
In order to meet the constantly growing energy needs, one of directions in the production of biofuels or bioethanol is using waste materials of agriculture, forestry and wood processing industry. In these bioprocesses, due to its many advantages, the producer microorganism is the yeast Saccharomyces cerevisiae. Although lignocellulosic materials are the source of simple sugars that are fermented into ethanol, the compounds that the yeast can ferment are obtained by pre-treatment during which growth and fermentation inhibitors, such as acetic and levulinic acid and 2-furaldehyde, are also formed. Since these compounds greatly impede bioethanol production, different studies have focused on the construction of new yeast strains that are more resistant to growth and fermentation inhibitors. Therefore, the influence of the overexpression of eight genes on the resistance of the yeast strain isolated from Sladorana d.o.o. Županja, to individual growth inhibitors, was investigated in this paper. The obtained results suggest that overexpression of YAP1 and CTA1 genes contributes to yeast resistance to levulinic acid and 2-furaldehyde and that yeast resistance to 2-furaldehyde is enhanced by overexpression of ATR1 and GSH1 genes.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:849682
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
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Created on2018-07-19 11:41:59