Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke

Jurković, Marko

prikaz prve stranice dokumenta Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke

undergraduate thesis

Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke

Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:745744

Jurković, Marko

University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

APA 6th Edition

Jurković, M. (2018). Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:745744

MLA 8th Edition

Jurković, Marko. "Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:745744

Chicago 17th Edition

Harvard

Jurković, M. (2018). 'Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:745744

Vancouver

Jurković M. Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 November 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:745744

IEEE

M. Jurković, "Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:745744

Cite this item: https://urn.nsk.hr/urn:nbn:hr:159:745744

Metadata

Title	Konstrukcija seta diploidnih sojeva kvasca Saccharomyces cerevisiae u svrhu uspostave semi-kvantitativne western blot metode za analizu proteina stanične stijenke
Title (english)	Construction of yeast Saccharomyces cerevisiae diploid strains for the purpose of developing a semi-quantative western blot method for analysis of cell wall proteins
Author	Marko Jurković
Mentor	Igor Stuparević (mentor)
Granter	University of Zagreb Faculty of Food Technology and Biotechnology (Department of Chemistry and Biochemistry) (Laboratory for Biochemistry) Zagreb
Defense date and country	2018-07-16, Croatia
Scientific / art field, discipline and subdiscipline	BIOTECHNICAL SCIENCES Biotechnology
Abstract	Stanična stijenka kvasca Saccharomyces cerevisiae određuje morfološke karakteristike stanice, kao što su oblik i čvrstoća, te doprinosi integritetu stanice tijekom rasta, diobe i stresnih okolišnih uvjeta. Građena je najvećim dijelom od polisaharida glukana i hitina te proteina. Cilj ovoga rada bio je uspostava semi-kvantitativne western blot metode za analizu proteina stanične stijenke. Prvo su konstruirani haploidni sojevi kvasca oba tipa parenja kojima je gen BGL2 označen hemaglutininskom sekvencom. Potom su iz ovih sojeva i haploidnih sojeva divljeg tipa procesom parenja konstruirani diploidni sojevi kojima su jedna, odnosno obje kopije gena BGL2 označene. Između proteinskih ekstrakata konstruiranih diploidnih sojeva detektirana je jasna razlika u intenzitetu signala vrpce proteina stanične stijenke Bgl2-HA, čime je pokazano da je provedena semi-kvantitativna western blot metoda pogodna za relativnu kvantifikaciju proteina Bgl2-HA.
Abstract (english)	Cell wall of yeast Saccharomyces cerevisiae defines morphological features of the cell, such as shape and rigidity, and is a key factor in cell integrity during growth, mitosis and exposure to stressful environmental factors. Major components of the cell wall are polysaccharides glucan and chitin, as well as proteins. The goal of this thesis was to develop a semi-quantitative western blot method for analysis of cell wall proteins. First, haploid yeast strains of both mating types in which BGL2 gene was tagged by hemagglutinin sequence were constructed. These strains and wild type haploid strains were then mated to construct a set of diploid strains in which one or both copies of BGL2 gene were tagged. Protein extracts from this set of diploid strains displayed a distinct difference in signal intensity of cell wall protein Bgl2-HA band, thereby showing that developed semi-quantitative western blot method is suitable for relative quantification of Bgl2-HA protein.
Keywords
Keywords (english)
Language	croatian
URN:NBN	urn:nbn:hr:159:745744
Study programme	Title: Food Technology Study programme type: university Study level: undergraduate Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka prehrambene tehnologije)
Type of resource	Text
File origin	Born digital
Access conditions	Open access
Terms of use
Created on	2018-08-29 07:31:56

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