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undergraduate thesis
Imunoenzimska metoda u određivanju alergena mlijeka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:695954
Horvatović, Matea
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Quality Control
Laboratory for Food Quality Control

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Horvatović, M. (2018). Imunoenzimska metoda u određivanju alergena mlijeka (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:695954

Horvatović, Matea. "Imunoenzimska metoda u određivanju alergena mlijeka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:695954

Horvatović, Matea. "Imunoenzimska metoda u određivanju alergena mlijeka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:695954

Horvatović, M. (2018). 'Imunoenzimska metoda u određivanju alergena mlijeka', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:695954

Horvatović M. Imunoenzimska metoda u određivanju alergena mlijeka [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 May 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:695954

M. Horvatović, "Imunoenzimska metoda u određivanju alergena mlijeka", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:695954

Metadata
TitleImunoenzimska metoda u određivanju alergena mlijeka
Title (english)Immunoenzyme method for milk allergens determination
AuthorMatea Horvatović
MentorKsenija Marković (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Quality Control)
(Laboratory for Food Quality Control)
Zagreb
Defense date and country2018-09-10, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Imunoenzimska ELISA (engl. Enzyme-Linked ImmunoSorbent Assay) metoda jednostavna je i brza metoda kojom se određuje prisutnost i količina antigena. Budući da se radi o vrlo specifičnoj reakciji antigen-antitijelo, u ovome radu korištena je u cilju određivanja alergena mlijeka u osam uzoraka keksa i čajnih peciva, od čega u četiri uzorka iz trgovačkih lanaca te četiri uzorka iz pekarnica sa zagrebačkog područja. U uzorcima keksa i čajnih peciva iz trgovačkih lanaca nisu detektirani alergeni mlijeka, dok se udio alergena mlijeka određen u tri od četiri analizirana uzorka iz pekarnica kretao u rasponu od 0,24 mgkg⁻¹ do 0,32 mgkg⁻¹. Rezultati imunoenzimskih analiza također su uspoređeni s informacijama o hrani naznačenim na analiziranim proizvodima. Uočeno je kako dva uzorka keksa i čajnih peciva iz trgovačkih lanaca u okviru informacija o hrani sadrže navod "može sadržavati" vezan uz prisutnost alergena mlijeka, iako alergeni mlijeka imunoenzimskim analizama nisu detektirani, dok jedan od uzoraka iz pekarnica u kojem su detektirani alergeni mlijeka ne sadrži navod o prisutnosti alergena mlijeka.
Abstract (english)
Immunoenzyme ELISA method (Enzyme-Linked ImmunoSorbent Assay) is a simple and rapid method for detecting and quantitating antigens. Since there is highly specific antigen-antibody reaction, in this study it is used for detecting and quantitating milk allergens in eight samples of biscuits and tea biscuits, four samples from supermarkets and four samples from bakeries on Zagreb market. In samples of biscuits and tea biscuits from supermarkets, milk allergens were not detected, while milk allergens were determined in three of four samples from bakeries in the range from 0,24 mgkg⁻¹ to 0,32 mgkg⁻¹. The results of immunoenzyme analysis were compared with food labels that were provided on products used in this research. It was noticed that two samples of biscuits and tea biscuits from supermarkets have statement „may contain traces of milk products“ on their food labels, but milk allergens were not detected with immunoezyme analysis, while one of the samples from bakeries in which milk allergens were detected didn't provide any information of the presence of milk allergens.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:695954
Study programmeTitle: Nutrition
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-09-12 07:24:44