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undergraduate thesis
Biološka aktivnost ekstrakta komine grožđa pripravljenog primjenom eutektičkog otapala
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:380211
Hrestak, Dora
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Hrestak, D. (2018). Biološka aktivnost ekstrakta komine grožđa pripravljenog primjenom eutektičkog otapala (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:380211

Hrestak, Dora. "Biološka aktivnost ekstrakta komine grožđa pripravljenog primjenom eutektičkog otapala." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:380211

Hrestak, Dora. "Biološka aktivnost ekstrakta komine grožđa pripravljenog primjenom eutektičkog otapala." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:380211

Hrestak, D. (2018). 'Biološka aktivnost ekstrakta komine grožđa pripravljenog primjenom eutektičkog otapala', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:380211

Hrestak D. Biološka aktivnost ekstrakta komine grožđa pripravljenog primjenom eutektičkog otapala [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 December 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:380211

D. Hrestak, "Biološka aktivnost ekstrakta komine grožđa pripravljenog primjenom eutektičkog otapala", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:380211

Metadata
TitleBiološka aktivnost ekstrakta komine grožđa pripravljenog primjenom eutektičkog otapala
Title (english)Biological activity of grape pomace extract prepared by using deep eutectic solvent
AuthorDora Hrestak
MentorKristina Radošević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2018-08-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Brojna epidemiološka ispitivanja ukazuju na potencijalne pozitivne učinke kemijskih spojeva iz komine gožđa na ljudsko zdravlje. Posljednjih godina za njihovu ekstrakciju, kao alternativa konvencionalnim, predlažu se zelena eutektična otapala. U ovom radu ekstrakti komine grožđa pripremljeni su uz pomoć zakiseljenog etanola (GPEtOH) te kolin-klorid:citratne kiseline (GPChCit). Nadalje, pripremljenim ekstraktima ispitana je biološka aktivnost na dvijema staničnim linijama (HeLa i HepG2), spektrofotometrijskom metodom određen je udio ukupnih polifenolnih spojeva, a ORAC metodom određen je antioksidacijski kapacitet ekstrakata. Ekstrakt komine grožđa pripravljen uz pomoć eutektičnog otapala imao je veći antioksidacijski kapacitet i sadržaj polifenola od onog ekstrahiranog zakiseljenim etanolom. Ispitivanjem antiproliferativnog djelovanja in vitro na stanicama uočeno je inhibitorno djelovanje oba ekstrakta, koje je izraženije na HeLa stanice. Nije zapažena značajna razlika u djelovanju GPEtOH i GPChCit ekstrakta na rast HeLa i HepG2 stanica.
Abstract (english)
Numerous epidemiological studies indicate that chemical compounds isolated from grape pomace have potential positive effects on human health. Lately, as an alternative method for their extraction, green deep eutectic solvents have been proposed. In this study, grape pomace extracts were prepared using acidified ethanol (GPEtOH) and choline-chloride: citric acid (GPChCit). Furthermore, the biological activity of the extracts was tested on two cell lines (HeLa and HepG2), total polyphenols were analyzed spectrophotometrically and the antioxidant capacity was determined by the ORAC method. The grape pomace extract prepared by eutectic solvent have higher antioxidative capacity and higher amount of polyphenols than the one extracted by acidified ethanol. Investigation of antiproliferative activity in vitro on the cell lines showed the inhibitory effect for the both extracts, which was more pronounced in HeLa cells. There was no significant difference in the effect of GPEtOH and GPChCit extract on HeLa and HepG2 cell growth.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:380211
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-09-24 07:30:52