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undergraduate thesis
Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:028171
Debanić, Fran
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Biochemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Debanić, F. (2018). Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:028171

Debanić, Fran. "Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:028171

Debanić, Fran. "Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:028171

Debanić, F. (2018). 'Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 July 2024, https://urn.nsk.hr/urn:nbn:hr:159:028171

Debanić F. Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 July 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:028171

F. Debanić, "Heterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:028171

Metadata
TitleHeterologna ekspresija rekombinantnih enzima za razgradnju atrazina na površini stanične stijenke kvasca Saccharomyces cerevisiae
Title (english)Heterologous expression of the atrazine degradation pathway enzymes on the surface of yeast Saccharomyces cerevisiae cell
AuthorFran Debanić
MentorRenata Teparić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Biochemistry)
Zagreb
Defense date and country2018-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Kvasac Saccharomyces cerevisiae jedan je od najčešće korištenih mikroorganizama u biotehnologiji, biomedicini i raznim znanstvenim istraživanjima. U ovom radu kvasac se koristio za ekspresiju enzima za razgradnju atrazina koji potječu iz Pseudomonas sp. ADP. Atrazin je herbicid koji je zabranjen od strane EU jer zbog svog kumulativnog efekta izaziva onečišćenje okoliša. Metabolički put razgradnje atrazina se sastoji od šest enzimskih reakcija od kojih je svaka katalizirana s po jednim enzimom. Cilj rada je bio eksprimirati i imobilizirati na površini stanične stijenke kvasca S. cerevisiae prva četiri enzima metaboličkog puta razgradnje atrazina (AtzA, AtzB, AtzC i AtzD), a u tu svrhu korišteni su proteini stanične stijenke kvasca Pir4 i Ccw12 s kojima su ti enzimi bili genetički fuzionirani. Uspješnost ekspresije i imobilizacije rekombinantih proteina provjeravala se Western blot analizom proteina ekstrahiranih iz izoliranih staničnih stijenki kvasca. Osim toga ispitivala se i aktivnost eksprimiranih rekombinantih enzima u tekućem mediju i na krutoj podlozi u Petrijevim zdjelicama.
Abstract (english)
Yeast Saccharomyces cerevisiae is one of the most commonly used microorganisms in biotechnology, biomedicine and various scientific research. In this thesis yeast was used to express the atrazine degradation enzymes originated from Pseudomonas sp. ADP. Atrazine is a herbicide that is banned from the EU because of its cumulative effect on environmental pollution. The metabolism of atrazine degradation consists of six enzymatic reactions each of which is catalysed with one enzyme. The aim of this study was to express and immobilize the first four enzymes of the atrazine degradation pathway (AtzA, AtzB, AtzC and AtzD) on the surface of the S. cerevisiae cell. For this purpose, Pir4 and Ccw12 yeast cell wall proteins were genetically fused with these enzymes. Success of expression and immobilization of the recombinant proteins was verified by Western blot analysis of proteins extracted from isolated yeast cell walls. In addition, the activity of expressed recombinant enzymes in the liquid medium and on the solid substrate in Petri dishes was also investigated.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:028171
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
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Created on2018-09-24 07:56:17