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undergraduate thesis
Bakterije mliječne kiseline izolirane iz majčinog mlijeka
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:493257
Širić, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Širić, A. (2018). Bakterije mliječne kiseline izolirane iz majčinog mlijeka (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:493257

Širić, Ana. "Bakterije mliječne kiseline izolirane iz majčinog mlijeka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:493257

Širić, Ana. "Bakterije mliječne kiseline izolirane iz majčinog mlijeka." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:493257

Širić, A. (2018). 'Bakterije mliječne kiseline izolirane iz majčinog mlijeka', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 06 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:493257

Širić A. Bakterije mliječne kiseline izolirane iz majčinog mlijeka [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 June 06] Available at: https://urn.nsk.hr/urn:nbn:hr:159:493257

A. Širić, "Bakterije mliječne kiseline izolirane iz majčinog mlijeka", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:493257

Metadata
TitleBakterije mliječne kiseline izolirane iz majčinog mlijeka
Title (english)Lactic acid bacteria isolated from breast milk
AuthorAna Širić
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2018-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Majčino mlijeko sadrži brojne supstance, a uz to i žive bakterije koje koloniziraju neonatalni gastrointestinalni trakt (GIT) dojenčeta. Cilj ovog rada bio je izolirati bakterije mliječne kiseline (BMK) iz majčinog mlijeka. Izolacija je provedena uspješno, pri čemu je izolirano 60 novih bakterijskih sojeva kojima su ispitani genotipski profili bakterijskih izolata amplifikacijom DNA korištenjem univerzalne M13 (5’-GAGGGTGGCGGTTCT-3’) početnice. Ispitana je također prisutnost gena koji kodiraju za S-proteine u genomu sojeva izoliranih iz majčinog mlijeka. RAPD-PCR analiza produkata dobivenih sa specifičnim Usl početnicama je pokazala da svi ispitani sojevi BMK u genomu sadrže jedan ili više gena koji kodiraju za S-proteine. Rezultati SDS-PAGE pokazuju da nijedan uzorak površinskih proteina ne sadrži karakterističnu proteinsku vrpcu koja bi ukazala da ispitivani sojevi BMK eksprimiraju S-proteine.
Abstract (english)
Breast milk contains numerous substances and also alive bacteria colonizing neonatal gastrointestinal tract (GIT) of an infant. Purpose of this thesis was to isolate lactic acid bacteria (LAB) from breast milk. Isolation was carried out successfully, whereby 60 new bacterial strains were isolated and examined for genotypic profiles with DNA amplification using universal M13 (5’-GAGGGTGGCGGTTCT-3’) primer. Existence of genes which codes for S-proteins in genome of strains isolated from breast milk was also examined. RAPD-PCR analysis of products obtained with specific Usl primers showed that all tested LAB strains contain one or more genes encoding S-proteins in the genomes. Results indicate that neither sample of surface proteins does not contain characteristical protein band, which represents expression of S-proteins in examined LAB strains.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:493257
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-10-04 09:14:23