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undergraduate thesis
Preživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:025799
Stanečić, Željka
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Stanečić, Ž. (2018). Preživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:025799

Stanečić, Željka. "Preživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:025799

Stanečić, Željka. "Preživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:025799

Stanečić, Ž. (2018). 'Preživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 26 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:025799

Stanečić Ž. Preživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 June 26] Available at: https://urn.nsk.hr/urn:nbn:hr:159:025799

Ž. Stanečić, "Preživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:025799

Metadata
TitlePreživljavanje i morfologija tumorske stanične linije MCF-7 u prisustvu ekstrakata đumbira
Title (english)The viability and morphology of tumor MCF-7 cells in the presence of ginger extracts
AuthorŽeljka Stanečić
MentorVišnja Gaurina Srček (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2018-09-19, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
U suvremenom svijetu primjena biološki aktivnih tvari iz biljaka koje imaju blagotvoran učinak na organizam sve je raširenija. Za određivanje biološke aktivnosti spojeva iz prirodnih izvora često se koriste in vitro testovi koji uključuju uporabu različitih humanih staničnih linija. Pomoću in vitro testova najčešće se ispituje tzv. bazalna citotoksičnost kojom se određuje učinak ispitivane tvari na preživljavanje stanica u kulturi. U ovom radu provedeno je in vitro ispitivanje učinaka subkritičnih vodenih ekstrakata đumbira na tumorskoj staničnoj liniji MCF-7 te je određena vijabilnost stanica MTS metodom 48 sati nakon tretmana. Rezultati su pokazali inhibitorno djelovanje ekstrakta na preživljavanje stanica, a maksimalna inhibicija od 61,21% postignuta je tretiranjem stanica sa 100 mg/mL ekstrakta. Morfološke promjene u stanicama nakon tretmana praćene su primjenom svjetlosne i fluorescentne mikroskopije te su u korelaciji s rezultatima određivanja vijabilnosti stanica. Rezultati dobiveni u ovom istraživanju ukazuju na mogućnost primjene đumbira kao izvora biološki aktivnih spojeva.
Abstract (english)
Nowadays, use of biologically active substances from plant sources with beneficiary effects on human health is increasing. Biological potential of such substances is often studied by in vitro tests involving the use of human cell lines. In vitro tests are usually assessed basal cytotoxicity by determining the effects of the test substance on cell culture survival. In this study effects of subcritical water extract of ginger has been studied in vitro on human tumor cell line MCF-7. Cell viability was determined by MTS method after 48 hours of treatment. The results showed the inhibitory effect of extracts on cell survival, and maximum inhibition of 61,21% was achieved by treatment of cells with 100 mg/mL of extract. Morphological changes in cells after treatment are determined by light and fluorescence microscopy and correlated to the viability results. The obtained results of this study indicate the possibility of using ginger as a source of biologically active compounds.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:025799
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2018-10-10 09:14:03