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undergraduate thesis
Pojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:299267
Leitner, Nina
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biology and Microbial Genetics

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Leitner, N. (2019). Pojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:299267

Leitner, Nina. "Pojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:299267

Leitner, Nina. "Pojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:299267

Leitner, N. (2019). 'Pojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 28 June 2024, https://urn.nsk.hr/urn:nbn:hr:159:299267

Leitner N. Pojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 June 28] Available at: https://urn.nsk.hr/urn:nbn:hr:159:299267

N. Leitner, "Pojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:299267

Metadata
TitlePojačana ekspresija alela YAP1C620F povećava otpornost kvasca na octenu kiselinu i 2-furaldehid
Title (english)Overexpression of the YAP1C620F allele increases resistance of the yeast to acetic acid and 2-furaldehyde
AuthorNina Leitner
MentorAnamarija Štafa (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biology and Microbial Genetics)
Zagreb
Defense date and country2019-06-14, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Svjetske zalihe fosilnih goriva se smanjuju, a zbog onečišćenja okoliša uzrokovanog njihovom prekomjernom upotrebom potrebno je naći ekonomičniju i održiviju alternativu. Proizvodnja bioetanola pomoću kvasca Saccharomyces cerevisiae, koristeći obnovljivi izvor, lignocelulozne sirovine, jedna je od alternativa za proizvodnju goriva. Kvasac ne može direktno fermentirati lignoceluozne sirovine te ih je stoga potrebno prvo hidrolizirati kako bi se oslobodili fermentabilini šećeri, ali predtretmanom lignoceluloznih sirovina nastaju i inhibitori koji usporavaju ili onemogućuju rast mikroorganizama te se stoga mnoga istraživanja temelje na konstrukciji proizvodnih sojeva otpornih na inhibitore rasta. U ovome radu transformiran je soj kvasca Žup ura- plazmidom pSP-YAP1-C620F koji omogućuje pojačanu ekspresiju konstitutivno aktivnog alelaYAP1C620F, za kojeg je pokazano da povećava otpornost nekih sojeva kvasca S. cerevisiae na inhibitore rasta. Konstruirani soj Žup YAP1C620F pokazao je veću otpornost na octenu kiselinu i 2-furaldehid u usporedbi sa sojem koji ima pojačanu ekspresiju divljeg tipa gena YAP1.
Abstract (english)
World supplies of fossil fuels are decreasing and, due to the environmental pollution caused by their excessive use, it is necessary to find a more economical and a more sustainable alternative. Fermentation of renewable lignocellulose substrates to ethanol by the yeast Saccharomyces cerevisiae is one of the alternatives for fuel production. Since yeast cannot directly ferment lignocellulose substrates, it is first necessary to hydrolase lignocellulose in order to release fermentable sugars. Moreover, during the pretreatment of lignocellulose biomass, different inhibitors that slow down or inhibit growth of microorganisms are formed. Therefore, various attempts have been made to construct a production strain that will be resistant to those growth inhibitors. In this research, the yeast strain Žup ura- was transformed with the pSP-YAP1-C620F plasmid that enables overexpression of constitutively active gene YAP1C620F that, for some strains of the yeast S. cerevisiae, increases resistance to growth inhibitors. Constructed strain with overexpression of YAP1C620F allele indeed displayed increased resistance to acetic acid and 2-furaldehyde in comparison to a strain that has overexpression of the wild type YAP1 gene.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:299267
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
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Created on2019-07-12 09:03:02