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undergraduate thesis
Proizvodnja ekstracelularnih enzima pomoću gljive Trametes versicolor
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:053886
Dobrotić, Dominik
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Dobrotić, D. (2019). Proizvodnja ekstracelularnih enzima pomoću gljive Trametes versicolor (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:053886

Dobrotić, Dominik. "Proizvodnja ekstracelularnih enzima pomoću gljive Trametes versicolor." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:053886

Dobrotić, Dominik. "Proizvodnja ekstracelularnih enzima pomoću gljive Trametes versicolor." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:053886

Dobrotić, D. (2019). 'Proizvodnja ekstracelularnih enzima pomoću gljive Trametes versicolor', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 25 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:053886

Dobrotić D. Proizvodnja ekstracelularnih enzima pomoću gljive Trametes versicolor [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2025 January 25] Available at: https://urn.nsk.hr/urn:nbn:hr:159:053886

D. Dobrotić, "Proizvodnja ekstracelularnih enzima pomoću gljive Trametes versicolor", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:053886

Metadata
TitleProizvodnja ekstracelularnih enzima pomoću gljive Trametes versicolor
Title (english)Production of extracellular enzymes using fungus Trametes versicolor
AuthorDominik Dobrotić
MentorTonči Rezić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2019-07-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Proveden je saržni uzgoj gljive T. versicolor u tekućoj hranjivoj podlozi s ciljem optimiziranja proizvodnje ekstracelularnih lignocelulolitičkih enzima. Uzgoj se provodio u pet različitih hranjivih podloga sa tri različita izvora ugljika: glukoza, lipa i hrast. Izvori ugljika bili su sami u podlozi ili u kombinaciji s glukozom u ekvivalentnom masenom odnosu (lipa i glukoza; hrast i glukoza). Tijek biosinteze enzima pratio se mjerenjem enzimske aktivnosti lakaza, mangan peroksidaza i celobioza dehidrogenaza metodom mjerenja promjene apsorbancije o vremenu za određeni supstrat pri određenoj valnoj duljini. Hemicelulazna i celulazna aktivnost mjerila se u 18. danu uzgoja DNSA metodom. Hrast se pokazao kao dobar induktor sinteze lignolitičkih i hemicelulolitičkih enzima. Najoptimalnija hranjiva podloga za proizvodnju ekstracelularnih enzima bila je podloga sa kombiniranim izvorom ugljika, hrastom i glukozom, u kojoj je u 18. danu uzgoja dobivena lakazna aktivnost iznosila 0,658 U/mL, mangan peroksidazna aktivnosti iznosila 0,073 U/mL, ksilanazna aktivnost iznosila 1,635 U/mL, pektinazna aktivnost iznosila je 0,503 U/mL i arabinazna aktivnost iznosila je 0,689 U/mL.
Abstract (english)
Batch cultivation of fungus T. versicolor was conducted in the liquid growth medium in order to optimise the production of extracellular lignocelulolytic enzymes. Cultivation was conducted in five different growth medium with three different carbon sources: glucose, lime tree and oak. Carbon sources were either the only carbon source or in combination with glucose in equivalent mass ratio (lime and glucose; oak and glucose). The course of enzyme biosynthesis was monitored by measuring the enzyme activity of laccases, manganese peroxidases and cellobiose dehydrogenases by measuring the slope of the absorption of specified substrate at a specified wavelength. Hemicellulase and cellulase activity was measured during the 18th day of cultivation using the DNSA assay. Oak was shown to be a good inductor of ligninolytic and hemicellulolytic enzyme synthesis. The most optimal growth medium was the medium with the combined carbon sources, oak and glucose, at which during the 18th day of cultivation the obtained laccase activity was 0.658 U/ml, manganese peroxidase activity was 0.073 U/ml, xylanase activity was 1.635 U/ml, pectinase activity was 0.503 U/ml and arabinase activity was 0.689 U/ml.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:053886
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-07-16 10:04:42