prikaz prve stranice dokumenta Proteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica
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undergraduate thesis
Proteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. urn:nbn:hr:159:509418
Larva, Mirna
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Larva, M. (2019). Proteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica (Undergraduate thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:509418

Larva, Mirna. "Proteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:509418

Larva, Mirna. "Proteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica." Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2019. https://urn.nsk.hr/urn:nbn:hr:159:509418

Larva, M. (2019). 'Proteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica', Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 May 2024, https://urn.nsk.hr/urn:nbn:hr:159:509418

Larva M. Proteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica [Undergraduate thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2019 [cited 2024 May 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:509418

M. Larva, "Proteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica", Undergraduate thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2019. Available at: https://urn.nsk.hr/urn:nbn:hr:159:509418

Metadata
TitleProteinski hidrolizat uljne pogače lana kao dodatak mediju za uzgoj CHO stanica
Title (english)Flaxseed protein hydrolysate as addition to medium for cultivation of CHO cells
AuthorMirna Larva
MentorIgor Slivac (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2019-09-09, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Posljednjih nekoliko desetljeća pokušavaju se pronaći alternative korištenju seruma animalnog podrijetla u tehnologiji životinjskih stanica zbog određenih nedostataka poput pojave mogućih kontaminacija i povećanih troškova procesa pročišćavanja. Biljni proteinski hidrolizati koriste se stoga kao djelomična ili potpuna zamjena za serum budući da sadrže mnoge hranjive tvari i komponente nužne za uspješan rast i proliferaciju kulture životinjskih stanica. Cilj ovog rada je istražiti i ispitati učinak proteinskog hidrolizata uljne pogače lana dobivenog pomoću tri enzima: Alcalase, Neutrase i Protamex na rast i produktivnost CHO-DP 12 stanične linije u kemijski definiranom mediju bez seruma. Ispitani su učinci hidrolizata dodanih u medij u dvije različite koncentracije: 2 g L-1 i 0.5 g L-1. Istraživanje pokazuje da dodatak hidrolizata dobivenih Protamexom i Neutrasom u koncentraciji od 2 i 0,5 g L-1 povoljno utječe na rast, ali i na produktivnost CHO-DP12 stanica u odnosu na stanice uzgajane u mediju bez dodatka hidrolizata. Hidrolizati dobiveni hidrolizom pomoću enzima Alcalase, dodani u obje ispitivane koncentracije, uzrokuju smrt stanične linije.
Abstract (english)
Numerous researches have beed done during the last few decades in order to find alternatives for using the animal origin serum due to several deficits like possible contaminations or additional costs for purification. Plant derived protein hydrolysates are therefore used as a partial or complete substitute because of containing various nutriments and similar components needed for a successful growth and proliferation of an animal cell culture. The aim of this paper is to find and test the effect of the protein hydrolysate of flaxseed oil cake obtained by three enzymes, Alcalase, Neutrase and Protamex on cell growth and productivity of CHO DP-12 cell line in a chemically defined medium without serum. Moreover, the effect of the examined hydrolysate in media has been analysed in two different concentrations: 2 g L-1 and 0,5 g L-1. This research shows that the addition of two hydrolysates, which are obtained by Protamex and Neutrase in the 2 and 0,5 g L-1 concentration, has a positive impact on the growth and productivity of CHO-DP12 cells compared to the cells in a hydrolysate-free medium. Nevertheless, the hydrolysates obtained by Alcalase causes the starvation of cell lines in both examined concentrations.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:509418
Study programmeTitle: Biotechnology
Study programme type: university
Study level: undergraduate
Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) inženjer/inženjerka biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2019-09-23 08:21:01