prikaz prve stranice dokumenta Interaction of Parkinson s disease protein alpha-synuclein with lipid membranes
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master's thesis
Interaction of Parkinson s disease protein alpha-synuclein with lipid membranes
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:624427
Gulija, Mateja
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Chemistry and Biochemistry
Laboratory for Organic Chemistry

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Gulija, M. (2018). Interaction of Parkinson s disease protein alpha-synuclein with lipid membranes (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:624427

Gulija, Mateja. "Interaction of Parkinson s disease protein alpha-synuclein with lipid membranes." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:624427

Gulija, Mateja. "Interaction of Parkinson s disease protein alpha-synuclein with lipid membranes." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:624427

Gulija, M. (2018). 'Interaction of Parkinson s disease protein alpha-synuclein with lipid membranes', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 29 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:624427

Gulija M. Interaction of Parkinson s disease protein alpha-synuclein with lipid membranes [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 November 29] Available at: https://urn.nsk.hr/urn:nbn:hr:159:624427

M. Gulija, "Interaction of Parkinson s disease protein alpha-synuclein with lipid membranes", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:624427

Metadata
TitleInteraction of Parkinson s disease protein alpha-synuclein with lipid membranes
Title (croatian)Interakcija Parkinsonovog proteina alfa-sinukleina sa lipidnim membranama
AuthorMateja Gulija
MentorLidija Barišić (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Chemistry and Biochemistry)
(Laboratory for Organic Chemistry)
Zagreb
Defense date and country2018-12-11, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Alpha-synuclein is a soluble synaptic protein whose aggregation resulting in amyloid Lewy bodies formation within neurons is a pathological characteristic of Parkinson’s disease. It is believed that the interaction of alpha-synuclein with synaptic lipid vesicles is related to the protein function. The aim of this thesis is to characterize the interaction of alpha-synuclein with negatively charged vesicles of 1,2-dioleoyl–phosphatidyl-glycerol (DOPG) and 1,2-dioleoyl- phosphatidyl-serine (DOPS) and the consequences of such interactions on alpha-synuclein amyloid formation by using biophysical methods. It was detected that alpha-synuclein to phospholipid ratio modulates the kinetics of the protein aggregation. High concentrations of lipids (lipid:protein molar ratio above 40) induced alpha-helical structure in alpha-synuclein and repressed the protein aggregation whereas at low concentrations of lipids, alpha-synuclein has shown partial folding and fibril formation was stimulated.
Abstract (english)
Alfa-sinuklein je topljivi sinaptički protein čija je agregacija, kojom dolazi do tvorbe amiloidnih Lewy tijela unutar neurona, patološka karakteristika Parkinsonove bolesti. Vjeruje se da interakcija alfa-sinukleina sa sinaptičkim lipidnim vezikulama ima utjecaj na funkciju proteina. Cilj ovog rada je karakterizirati interakciju alfa-sinukleina s negativno nabijenim vezikulama priređenima iz 1,2-dioleoil-fosfatidil-glicerola (DOPG) i 1,2-dioleoil-fosfatidil-serina (DOPS) te posljedice takvih interakcija na formiranje alfa-sinuklein amiloida upotrebom biofizikalnih metoda. Otkriveno je da omjer alfa-sinukleina prema fosfolipidima modulira kinetiku agregacije alfa-sinukleina. Visoke koncentracije lipida (omjer lipida i proteina iznad 40) dovele su do tvorbe heličnih struktura i ograničenja fibrilacije alfa-sinukleina, dok je pri niskoj koncentraciji lipida, alfa-sinuklein pokazao djelomično smatanje te je stimulirano stvaranje fibrila.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:624427
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsAccess restricted to students and staff of home institution
Terms of useLicence In copyright icon
Created on2019-11-14 13:07:03