prikaz prve stranice dokumenta Izolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku
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master's thesis
Izolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:415637
Matanić, Josipa
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Food Engineering
Laboratory for drying Technologies and monitoring of biologically active compounds

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Matanić, J. (2018). Izolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:415637

Matanić, Josipa. "Izolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:415637

Matanić, Josipa. "Izolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:415637

Matanić, J. (2018). 'Izolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 21 December 2024, https://urn.nsk.hr/urn:nbn:hr:159:415637

Matanić J. Izolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 December 21] Available at: https://urn.nsk.hr/urn:nbn:hr:159:415637

J. Matanić, "Izolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:415637

Metadata
TitleIzolacija pigmenata iz alge Cystoseira primjenom ubrzane ekstrakcije otapalima pri povišenom tlaku
Title (english)Isolation of pigments from algae Cystoseira using pressurized liquid extraction
AuthorJosipa Matanić
MentorVerica Dragović Uzelac (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Food Engineering)
(Laboratory for drying Technologies and monitoring of biologically active compounds)
Zagreb
Defense date and country2018-09-24, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Cilj ovog istraživanja bio je odrediti masene udjele klorofila a, klorofila b i karotenoida u ekstraktima liofilizirane smeđe alge Cystoseira proizvedenim pomoću ubrzane ekstrakcije otapalima pri povišenom tlaku (PLE). Uz etanol kao ekstrakcijsko otapalo, ispitivani su sljedeći parametri ekstrakcije: (i) statičko vrijeme ekstrakcije (5, 10 i 15 min); (ii) broj ciklusa ekstrakcije (1 i 2); te (iii) temperatura (100 °C, 130 °C, 160 °C). Maseni udjeli navedenih pigmenata određeni su spektrofotometrijski, a utjecaji parametara ekstrakcije definirani su na temelju provedene statističke analize MANOVA. Maseni udjeli klorofila a određeni su u rasponu od 0,86 do 1,92 mg g-1 s.t., klorofila b od 0,09 do 1,19 mg g-1 s.t. te karotenoida od 0,36 do 0,70 mg g-1 s.t. Utvrđena je statistički značajna razlika u masenim udjelima svih pigmenata u ovisnosti o svim primjenjenim parametrima ekstrakcije, osim kod klorofila b, gdje broj ciklusa ekstrakcije nije statistički značajan parametar. Rezultati istraživanja pokazali su da se najveći prinosi pigmenata dobivaju pri slijedećim uvjetima ekstrakcije: klorofil a pri temperaturi ekstrakcije od 160 °C i jedan ciklus od 5 minuta; klorofil b i karotenoidi pri temperaturi ekstrakcije od 130 °C te jedan ciklus od 15 minuta.
Abstract (english)
The aim of this study was to determine mass fractions of chlorophyll a, chlorophyll b and carotenoids in extracts of lyophilized brown algae Cystoseira, produced by pressurized liquid extraction (PLE). Ethanol was the extraction solvent and the following extraction parameters were investigated: (i) static extraction time (5, 10 and 15 min); (ii) number of extraction cycles (1 and 2); and (iii) temperature (100 °C, 130 °C, 160 °C). Mass fractions of certain pigmentsin obtained extracts were determined spectrophotometrically and the effect of extraction parameters was defined by statistical analysis MANOVA. Mass fractions of chlorophyll a were ranged from 0.86 to 1.92 mg g-1 s.w., chlorophyll b from 0.09 to 1.19 mg g-1 s.w. and carotenoids from 0.36 to 0.70 mg g-1 s.w. A statistically significant difference was determined depending on all applied extraction parameters, except for chlorophyll b, where the number of extraction cycles was not a statistically significant parameter. The results of the research have shown that the highest pigment yields are obtained under the following extraction conditions: chlorophyll a at extraction temperature of 160 °C and one 5 minute cycle; chlorophyll b and carotenoids at extraction temperature of 130 °C and one 15 minutes cycle.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:415637
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-14 13:17:02