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master's thesis
New aspects of enzymes working in lignocellulose degradation
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:913919
Hanžek, Marija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Hanžek, M. (2017). New aspects of enzymes working in lignocellulose degradation (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:913919

Hanžek, Marija. "New aspects of enzymes working in lignocellulose degradation." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:913919

Hanžek, Marija. "New aspects of enzymes working in lignocellulose degradation." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:913919

Hanžek, M. (2017). 'New aspects of enzymes working in lignocellulose degradation', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 04 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:913919

Hanžek M. New aspects of enzymes working in lignocellulose degradation [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 August 04] Available at: https://urn.nsk.hr/urn:nbn:hr:159:913919

M. Hanžek, "New aspects of enzymes working in lignocellulose degradation", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:913919

Metadata
TitleNew aspects of enzymes working in lignocellulose degradation
Title (english)Novi aspekti enzima koji sudjeluju u razgradnji lignoceluloze
AuthorMarija Hanžek
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2017-12-08, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Fossil fuels have to be replaced by renewable energies (e.g. biomass) due to increasing global demand for energy. The discovery of LPMOs (lytic polysaccharide monooxygenases) was a breakthrough in understanding of how nature degrades biomass. The main obstacle in LPMO research is the lack of an easy to use spectroscopic activity assay. Accordingly, the aim of this thesis was to develop and validate an activity assay and concomitantly characterize the LPMO from the fungi Neurospora crassa. 2,6-DMP (2,6-dimethoxyphenol) as reductant in addition with the new cosubstrate H2O2 was proven to be the best chromogenic compound with a high extinction coefficient (24.8 mM-1cm-1) in the visible range (469 nm). The optimal LPMO activity was observed in the neutral to basic pH range. The lowest concentration of LPMO, which could be measured and distinguished from blank reactions, was ~0.0125 μM.
Abstract (english)
Zbog povećane globalne potražnje za energijom, fosilne izvore potrebno je zamijeniti obnovljivim izvorima energije (npr. biomasom). Otkriće LPMO (litičke polisaharid monooksigenaze) bilo je ključno za razumijevanje prirodne razgradnje biomase. Glavna prepreka u istraživanju LPMO je nedostatak jednostavnog protokola za istraživanje enzimske aktivnosti pomoću spektroskopije. Sukladno tome, cilj ovog rada bio je razviti i potvrditi protokol za utvrđivanje enzimske aktivnosti te istodobno okarakterizirati LPMO izoliranu iz plijesni Neurospora crassa. Dokazano je da je reduktant 2,6-DMP (2,6-dimetoksifenol), zajedno sa novootkrivenim kosupstratom H2O2, najbolja kromogenska komponenta sa visokim molarnim apsorpcijskim koeficijentom (24.8 mM-1cm-1) u vidljivom dijelu spektra (469 nm). Optimalna aktivnost LPMO je u neutralnom prema bazičnom pH području. Najniža koncentracija LPMO koja može biti izmjerena i koja se razlikuje od vrijednosti slijepe probe je ~0.0125 μM.
Keywords
Keywords (english)
Languageenglish
URN:NBNurn:nbn:hr:159:913919
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-15 11:24:41