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master's thesis
Biološka aktivnost ekstrakata nusproizvoda prehrambene industrije pripravljenih pomoću eutektičkih otapala
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. urn:nbn:hr:159:537625
Kelava, Ivana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Cell Technology, Application and Biotransformations

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

Cite this document

Kelava, I. (2017). Biološka aktivnost ekstrakata nusproizvoda prehrambene industrije pripravljenih pomoću eutektičkih otapala (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:537625

Kelava, Ivana. "Biološka aktivnost ekstrakata nusproizvoda prehrambene industrije pripravljenih pomoću eutektičkih otapala." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:537625

Kelava, Ivana. "Biološka aktivnost ekstrakata nusproizvoda prehrambene industrije pripravljenih pomoću eutektičkih otapala." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2017. https://urn.nsk.hr/urn:nbn:hr:159:537625

Kelava, I. (2017). 'Biološka aktivnost ekstrakata nusproizvoda prehrambene industrije pripravljenih pomoću eutektičkih otapala', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 23 November 2024, https://urn.nsk.hr/urn:nbn:hr:159:537625

Kelava I. Biološka aktivnost ekstrakata nusproizvoda prehrambene industrije pripravljenih pomoću eutektičkih otapala [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2017 [cited 2024 November 23] Available at: https://urn.nsk.hr/urn:nbn:hr:159:537625

I. Kelava, "Biološka aktivnost ekstrakata nusproizvoda prehrambene industrije pripravljenih pomoću eutektičkih otapala", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2017. Available at: https://urn.nsk.hr/urn:nbn:hr:159:537625

Metadata
TitleBiološka aktivnost ekstrakata nusproizvoda prehrambene industrije pripravljenih pomoću eutektičkih otapala
Title (english)Biological activity of extracts of food industry by-products prepared using eutectic solvents
AuthorIvana Kelava
MentorKristina Radošević (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Cell Technology, Application and Biotransformations)
Zagreb
Defense date and country2017-12-07, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Nusproizvodi prehrambene industrije često predstavljaju problem zbog zbrinjavanja, ali su ujedno i bogat izvor biološki vrijednih spojeva, kao što su fitokemikalije. Posljednjih se godina eutektička otapala (DES) istražuju kao alternativna otapala za zelenu ekstrakciju, a u ovom radu su korištena za ekstrakciju biološki aktivnih spojeva iz biljnog materijala. Pripremljeni su ekstrakti komine grožđa, komine masline i prešane lanene pogače pri prethodno optimiranim uvjetima s eutektičkim otapalima i konvencionalnim otapalima. Nadalje, pripremljenim ekstraktima spektrofotometrijskom metodom je određen udio polifenolih spojeva, antioksidacijski kapacitet ORAC metodom te je ispitana biološka aktivnost na trima staničnim linijama (HeLa, MCF-7, HEK293T). Najveći udio polifenolnih spojeva ekstrahiran je iz komine grožđa s eutektičkim otapalom kolin-klorid:limunska kiselina, a isti ekstrakt imao je najveći antioksidacijski kapacitet te najizraženiji inhibitorni učinak na stanične linije. Tretiranim humanim tumorskim staničnim linijama određen je i tip stanične smrti te je indukcija apoptoze izražena nakon tretmana ekstraktom komine grožđa pripremljenog u zakiseljenom etanolu.
Abstract (english)
Recurring problem in food industry is disposal of by-products which are also a rich source of biologically active compounds, such as phytochemicals. In recent years deep eutectic solvents (DES) are investigated as alternative solvents for green extraction and in this paper they are used for extraction of biologically active compounds from plant materials. Extracts from grape pomace, olive pomace and flaxseed pressed cake were prepared in optimized conditions with eutectic and conventional solvents. Furthermore, total polyphenols were analyzed using spectrophotometric method, antioxidant capacity was determined using ORAC method and biological activity was tested on three cell lines (HeLa, MCF-7, HEK93T). Grape pomace in DES exhibited the highest value of polyphenols, the highest antioxidant activity and the most prominent inhibitory effect on cell lines. Type of cell death was determined for treated human tumor cell lines and the induction of apoptosis was most significant after treatment with acidified ethanol.
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:537625
Study programmeTitle: Molecular Biotechnology
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka molekularne biotehnologije (magistar/magistra inženjer/inženjerka molekularne biotehnologije)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-15 11:28:51