prikaz prve stranice dokumenta Improvement of plasmid stability in E. coli for industrial production
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master's thesis
Improvement of plasmid stability in E. coli for industrial production
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:234166
Vlahović, Lukrecija
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Vlahović, L. (2018). Improvement of plasmid stability in E. coli for industrial production (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:234166

Vlahović, Lukrecija. "Improvement of plasmid stability in E. coli for industrial production." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:234166

Vlahović, Lukrecija. "Improvement of plasmid stability in E. coli for industrial production." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:234166

Vlahović, L. (2018). 'Improvement of plasmid stability in E. coli for industrial production', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:234166

Vlahović L. Improvement of plasmid stability in E. coli for industrial production [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:234166

L. Vlahović, "Improvement of plasmid stability in E. coli for industrial production", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:234166

Metadata
TitleImprovement of plasmid stability in E. coli for industrial production
Title (english)Povećanje stabilnosti plazmida u E. coli za industrijsku proizvodnju
AuthorLukrecija Vlahović
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2018-10-26, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
E. coli is most common host for recombinant protein production. Majority of the expression systems based on it depend on attendance of at least one plasmid that needs to be segregated into dividing cells during growth. Segregational plasmid stability is one of the major issues of industrial scale production of recombinant proteins during which conventional method of adding antibiotics should be excluded. One of the alternatives is to use auxotrophic mutants transformed with plasmid that contains gene of interest and gene that complements the mutation. In this thesis mutant strain of E coli with deleted gene for triosephosphate isomerase, transformed with plasmid which complements mutation and has resistance to antibiotic, is used. Its growth and segregational stability of the plasmid is compared to the transformed parent strain, cultivated in medium with and without antibiotic. In addition, new auxotrophic strain, with lower probability of creating revertants is created.
Abstract (croatian)
E. coli je najčešći domaćin za proizvodnju rekombinantnih proteina. Većina ekspresijskih sistema baziranih na njoj ovisi o pristutnosti barem jednog plazmida koji se treba pravilno rasporediti u stanice kćeri tijekom diobe. Segregacijska stabilnost plazmida je jedan od najvećih problema tijekom industrijske proizvodnje rekombinantnih proteina tijekom koje je potrebno izbjeći uobičajenu metodu dodatka antibiotika kulturi, a jedan od alternativnih načina je upotreba auksotrofnih mutanata transformiranih plazmidom koji uz gen od interesa sadrži gen koji komplementira mutaciju soja. U ovom radu korišten je mutiran soj E. coli s deletiranim genom za trioza fosfat izomerazu bez kojeg bakterija ne može rasti na glicerolu. Soj je transfomiran plazmidom koji komplementira mutaciju te ima rezistenciju na antibiotik. Rast mutiranog soja i segregacijska stabilnost plazmida uspoređeni su sa roditeljskim, ne mutiranim sojem, transformiranim istim plazmidom, te uzgajanim u mediju sa i bez antibiotika. Uz to, kreiran je novi auksotrofni soj, sa smanjenom mogućnošću pojave revertanata
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:159:234166
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence Attribution-NoDerivatives 4.0 International (CC BY-ND 4.0) icon
Created on2019-11-26 12:47:48