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master's thesis
Cultivation and characterization of E. coli protein – secretion strains
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:400382
Jurić, Laura
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Jurić, L. (2018). Cultivation and characterization of E. coli protein – secretion strains (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:400382

Jurić, Laura. "Cultivation and characterization of E. coli protein – secretion strains." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:400382

Jurić, Laura. "Cultivation and characterization of E. coli protein – secretion strains." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:400382

Jurić, L. (2018). 'Cultivation and characterization of E. coli protein – secretion strains', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 13 January 2025, https://urn.nsk.hr/urn:nbn:hr:159:400382

Jurić L. Cultivation and characterization of E. coli protein – secretion strains [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2025 January 13] Available at: https://urn.nsk.hr/urn:nbn:hr:159:400382

L. Jurić, "Cultivation and characterization of E. coli protein – secretion strains", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:400382

Metadata
TitleCultivation and characterization of E. coli protein – secretion strains
Title (english)Uzgoj i karakterizacija E. coli protein – sekrecijskih sojeva
AuthorLaura Jurić
MentorBožidar Šantek (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Biochemical Engineering, Industrial Microbiology and Malting and Brewing Technology)
Zagreb
Defense date and country2018-07-13, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Biotechnology
Abstract
Escherichia coli is one of the most commonly used microorganisms for production of recombinant proteins. The vast majority of proteins are produced intracellular, either in cytoplasm or periplasm, so that obtaining a biologically active proteins represents a challenge. With extracellular production of proteins, would be avoided cell disruption, ease protein yielding and purification. One of the approaches, applied and investigated in this thesis, is using so-called leaky mutants. Due to mutations in genes encoding for proteins of the cell membrane, these mutants show an increased protein release from periplasm to nutrient medium after protein has been translocated by using natural bacterial translocation systems. Two E. coli knock-out strains have been cultivated first in flasks (without and with pAppA plasmid) and then in bioreactors. Strains have been characterized regarding to their growth, protein production and secretion ability, using phytase as reporter protein.
Abstract (croatian)
Escherichia coli je jedan od najčešće korištenih mikroorganizama za proizvodnju rekombinantnih proteina. Veliku većinu proteina proizvodi intracelularno, bilo u citoplazmi ili u periplazmi, tako da dobivanje biološki aktivnih proteina predstavlja izazov. Ekstracelularnom proizvodnjom proteina izbjeglo bi se mehaničko razbijanje stanica, olakšala izolacija i pročišćavanje proteina. Jedan od pristupa, primjenjen i istražen u ovome radu, je upotreba takozvanih leaky mutanata. Zbog mutacija u genima koji kodiraju za proteine stanične stijenke, ovi mutanti bi trebali pokazivati povećanu sposobnost izlučivanja proteina iz periplazme u hranjivu podlogu nakon translokacije proteina prirodno prisutnim bakterijskim translokacijskim mehanizmima. Dva knock-out soja bakterije E. coli su kultivirani najprije u tikvicama (bez i s pAppA plazmidom) a zatim i u bioreaktorima. Kultivirani sojevi su karakterizirani obzirom na rast, koncentraciju proizvedenog proteina i sposobnost sekrecije koristeći fitazu kao reporter protein.
Keywords
Keywords (croatian)
Languageenglish
URN:NBNurn:nbn:hr:159:400382
Study programmeTitle: Bioprocess Engineering
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva (magistar/magistra inženjer/inženjerka bioprocesnog inženjerstva)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-26 12:54:57