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master's thesis
Određivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS)
Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. urn:nbn:hr:159:928719
Oguić, Ana
University of Zagreb
Faculty of Food Technology and Biotechnology
Department of Biochemical Engineering
Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology

Institutional repository: Repository of the Faculty of Food Technology and Biotechnology

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Oguić, A. (2018). Određivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS) (Master's thesis). Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology. Retrieved from https://urn.nsk.hr/urn:nbn:hr:159:928719

Oguić, Ana. "Određivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS)." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:928719

Oguić, Ana. "Određivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS)." Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, 2018. https://urn.nsk.hr/urn:nbn:hr:159:928719

Oguić, A. (2018). 'Određivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS)', Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, accessed 27 August 2024, https://urn.nsk.hr/urn:nbn:hr:159:928719

Oguić A. Određivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS) [Master's thesis]. Zagreb: University of Zagreb, Faculty of Food Technology and Biotechnology; 2018 [cited 2024 August 27] Available at: https://urn.nsk.hr/urn:nbn:hr:159:928719

A. Oguić, "Određivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS)", Master's thesis, University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, 2018. Available at: https://urn.nsk.hr/urn:nbn:hr:159:928719

Metadata
TitleOdređivanje rezidua cefalosporina u mišiću goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS)
Title (english)Determination of cephalosporin residue in cattle, pigs, poultry and fish muscle using the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC/Q-TOF-MS)
AuthorAna Oguić
MentorBlaženka Kos (mentor)
GranterUniversity of Zagreb
Faculty of Food Technology and Biotechnology
(Department of Biochemical Engineering)
(Laboratory for Antibiotic, Enzyme, Probiotic and Starter Cultures Technology)
Zagreb
Defense date and country2018-09-20, Croatia
Scientific / art field,
discipline and subdiscipline		BIOTECHNICAL SCIENCES
Nutrition
Abstract
Neadekvatna uporaba antibiotika u humanoj i veterinarskoj medicini dovodi do razvoja antimikrobne rezistencije, širenja otpornosti mikroorganizma te pojave njihovih ostataka u namirnicama životinjskog porijekla. Jedna od najčešće primijenjenih vrsta antibiotika su cefalosporini. Da bi ih adekvatno identificirali i kvantificirali bilo je potrebno razviti metodu i ispitati utjecaj metode za identifikaciju rezidua cefalosporina u mišićima goveda, svinje, peradi i ribe primjenom tekućinske kromatografije ultravisoke djelotvornosti sa spektrometrijom masa i analizatorom vremena preleta (UHPLC/Q-TOF-MS), što je ujedno i glavni cilj ovog rada. Također, validirana metoda za određivanje cefalosporina u mišićima različitih životinjskih vrsta visoke je specifičnosti, točnosti i preciznosti te zadovoljava kriterije o provođenju analitičkih metoda i tumačenju rezultata prema važećoj Uredbi komisije 2002/657/EZ. Metoda je primijenjena u rutinskoj analizi na Hrvatskom veterinarskom institutu. Na prisustvo 9 najčešćih cefalosporina testirana su 42 uzorka pri čemu nije pronađen ni jedan pozitivan uzorak.
Abstract (english)
Inadequate use of antibiotics in human and veterinary medicine leads to the development of antimicrobial resistance, the spread of microorganism resistance and the appearance of their residues in food of animal origin. One of the most frequently used types of antibiotics are cephalosporins. In order to identify and quantify, it was necessary to develop a method and to examine the effect of the method for identifying the residual cephalosporin residues in cattle, pigs, poultry and fish muscles using ultra-high performance liquid chromatography and high resolution mass spectrometry (UHPLC/Q-TOF-MS), which is also the main objective of this paper. Validated method for the determination of cephalosporin in the muscles of different animal species is of high specificity, accuracy and precision and meets the criteria for the conduct of analytical methods and interpretation of results under the current Commission Regulation 2002/657 / EC. The method was applied in a routine analysis at the Croatian Veterinary Institute. In the presence of 9 most common cephalosporins, 42 samples were tested and no positive samples were found
Keywords
Keywords (english)
Languagecroatian
URN:NBNurn:nbn:hr:159:928719
Study programmeTitle: Nutrition
Study programme type: university
Study level: graduate
Academic / professional title: magistar/magistra nutricionizma (magistar/magistra nutricionizma)
Type of resourceText
File originBorn digital
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2019-11-26 13:52:34